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评估脐带血来源的血小板裂解物和乏血小板血浆在角膜伤口愈合应用中的再生潜力：对角膜上皮细胞的体外比较研究。

Evaluation of the Regenerative Potential of Platelet-Lysate and Platelet-Poor Plasma Derived from the Cord Blood Units in Corneal Wound Healing Applications: An In Vitro Comparative Study on Corneal Epithelial Cells.

作者信息

Mallis Panagiotis, Michalopoulos Efstathios, Sarri Eirini Faidra, Papadopoulou Elena, Theodoropoulou Vasiliki, Katsimpoulas Michalis, Stavropoulos-Giokas Catherine

机构信息

Hellenic Cord Blood Bank (HCBB), Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou, 115 27 Athens, Greece.

Experimental Surgery Unit, Center of Clinical, Experimental Surgery and Translational Research, Βιοmedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou, 115 27 Athens, Greece.

出版信息

Curr Issues Mol Biol. 2022 Sep 22;44(10):4415-4438. doi: 10.3390/cimb44100303.

DOI:10.3390/cimb44100303
PMID:36286018
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9600746/
Abstract

BACKGROUND

Cord blood platelet lysate (CB-PL) and cord blood platelet poor plasma (CB-PPP) have been applied with success in wound healing applications. Pathologies such as Sjogrens's Syndrome (SS) and chronic graft versus host disease (cGVHD) can lead to severe ophthalmology issues. The application of CB-PL and CB-PPP may be strongly considered for damaged cornea healing. This study aimed to the evaluation of the beneficial properties of CB-PL and CB-PPP in corneal wound healing applications.

METHODS

Initially, the CB-PL and CB-PPP were produced from donated cord blood units (CBUs), followed by biochemical analysis. Corneal epithelial cells (CECs) were isolated from wistar rats and then cultured with medium containing 20% / either of CB-PL or CB-PPP. To define the impact of CB-PL and CB-PPP, biochemical, morphological analysis, scratch-wound assays, and immunoassays in CECs were performed.

RESULTS

CB-PL and CB-PPP were characterized by good biochemical parameters, regarding their quality characteristics and biomolecule content. CECs' morphological features did not change after their cultivation with CB-PL or CB-PPP. A scratch wound assay and molecular analysis of CECs expanded with CB-PL indicated higher migratory capacity compared to those cultured with CB-PPP.

CONCLUSION

CB-PL and CB-PPP exhibited good properties with respect to cell migration and proliferation, and could be considered an alternative source for eye drop production, to possibly be used in cornea wound healing applications.

摘要

背景

脐带血血小板裂解物（CB-PL）和脐带血少血小板血浆（CB-PPP）已成功应用于伤口愈合。诸如干燥综合征（SS）和慢性移植物抗宿主病（cGVHD）等病症可导致严重的眼科问题。对于受损角膜愈合，可强烈考虑应用CB-PL和CB-PPP。本研究旨在评估CB-PL和CB-PPP在角膜伤口愈合应用中的有益特性。

方法

首先，从捐赠的脐带血单位（CBUs）中制备CB-PL和CB-PPP，随后进行生化分析。从Wistar大鼠分离角膜上皮细胞（CECs），然后用含有20% CB-PL或CB-PPP的培养基进行培养。为了确定CB-PL和CB-PPP的影响，对CECs进行了生化、形态学分析、划痕试验和免疫测定。

结果

CB-PL和CB-PPP在质量特征和生物分子含量方面具有良好的生化参数。CECs在用CB-PL或CB-PPP培养后其形态特征未发生变化。与用CB-PPP培养的CECs相比，用CB-PL培养的CECs的划痕试验和分子分析表明其迁移能力更高。

结论

CB-PL和CB-PPP在细胞迁移和增殖方面表现出良好特性，可被视为眼药水生产的替代来源，可能用于角膜伤口愈合应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/a0869e749b05/cimb-44-00303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/e4a20c8a0474/cimb-44-00303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/66696da90f07/cimb-44-00303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/a3214ca945f0/cimb-44-00303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/7c0cabbd014f/cimb-44-00303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/b4b69784c7d9/cimb-44-00303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/a0869e749b05/cimb-44-00303-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/e4a20c8a0474/cimb-44-00303-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/66696da90f07/cimb-44-00303-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/a3214ca945f0/cimb-44-00303-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/7c0cabbd014f/cimb-44-00303-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/b4b69784c7d9/cimb-44-00303-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b39a/9600746/a0869e749b05/cimb-44-00303-g006.jpg

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