Yedier Serdar, Kontaş Yalçınkaya Seda, Bostanci Derya, Polat Nazmi
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Ordu University, Ordu, Turkey.
Department of Fisheries Technology Engineering, Fatsa Faculty of Marine Sciences, Ordu University, Ordu, Turkey.
Anat Histol Embryol. 2023 Mar;52(2):279-288. doi: 10.1111/ahe.12881. Epub 2022 Oct 26.
Otoliths are calcareous anatomical structures in the inner ear of fishes, and they can be used in the discrimination of fish species and stocks due to their species-specific shape. Sagittal otoliths in the Lepidorhombus whiffiagonis and Lepidorhombus boscii, two flatfish species of the same genus distributed in the Aegean Sea, were compared using morphology, morphometry and contour shape analyses. Blind and eyed side otoliths of Lepidorhombus species were evaluated separately due to their morphological features and statistical differences in morphometric measurements (p < 0.05). Four analysis groups were formed: blind side otolith of L. whiffiagonis, eyed side otolith of L. whiffiagonis, blind side otolith of L. boscii and eyed side otolith of L. boscii. Morphometric differences in otolith shape were performed by canonical discriminant analysis, and the first discriminant axis explained 97.4% (Wilks λ = 0.270) of the variance between the groups, and the second axis explained 2.3% (Wilks λ = 0.620). Classification success between Lepidorhombus species based on CDA is 100%, and the overall CDA classification score between groups is 76.5%. The wavelet functions obtained in the contour analysis showed high variability in the anterior, posterior-dorsal, antero-dorsal and ventral regions of the otoliths among these four analysis groups. Consequently, it is thought that this study will contribute to the taxonomic classification of fish with the morphological and morphometric differences in the blind and eyed side otoliths and the analyses made with the high discrimination success obtained in Lepidorhombus species.
耳石是鱼类内耳中的钙质解剖结构，由于其具有物种特异性形状，可用于区分鱼类物种和种群。对分布于爱琴海的同属两种鲽形目鱼类——惠氏菱鲆和博氏菱鲆的矢耳石进行了形态学、形态测量学和轮廓形状分析比较。由于菱鲆属鱼类盲侧和眼侧耳石的形态特征及形态测量统计差异（p < 0.05），对其分别进行了评估。形成了四个分析组：惠氏菱鲆盲侧耳石、惠氏菱鲆眼侧耳石、博氏菱鲆盲侧耳石和博氏菱鲆眼侧耳石。通过典型判别分析对耳石形状的形态测量差异进行分析，第一判别轴解释了组间方差的97.4%（威尔克斯λ = 0.270），第二轴解释了2.3%（威尔克斯λ = 0.620）。基于典型判别分析的菱鲆属物种间分类成功率为100%，组间总体典型判别分析分类得分是76.5%。轮廓分析中获得的小波函数显示，在这四个分析组的耳石前部、后背部、前背部和腹侧区域存在高度变异性。因此，认为本研究将通过盲侧和眼侧耳石的形态和形态测量差异以及在菱鲆属物种中获得的高判别成功率分析，为鱼类的分类学分类做出贡献。
