线性微断层合成扫描仪的在线校准

Online Calibration of a Linear Micro Tomosynthesis Scanner.

作者信息

Bahar Piroz, Nguyen David, Wang Muyang, Mazilu Dumitru, Bennett Eric E, Wen Han

机构信息

Laboratory of Imaging Physics, Biochemistry and Biophysics Center, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.

出版信息

J Imaging. 2022 Oct 21;8(10):292. doi: 10.3390/jimaging8100292.

DOI:10.3390/jimaging8100292

PMID:36286386

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9604648/

Abstract

In a linear tomosynthesis scanner designed for imaging histologic samples of several centimeters size at 10 µm resolution, the mechanical instability of the scanning stage (±10 µm) exceeded the resolution of the image system, making it necessary to determine the trajectory of the stage for each scan to avoid blurring and artifacts in the images that would arise from the errors in the geometric information used in 3D reconstruction. We present a method for online calibration by attaching a layer of randomly dispersed micro glass beads or calcium particles to the bottom of the sample stage. The method was based on a parametric representation of the rigid body motion of the sample stage-marker layer assembly. The marker layer was easy to produce and proven effective in the calibration procedure.

摘要

在一台用于对几厘米大小的组织学样本进行成像、分辨率为10微米的线性断层合成扫描仪中，扫描台的机械不稳定性（±10微米）超过了图像系统的分辨率，因此有必要在每次扫描时确定扫描台的轨迹，以避免因三维重建中使用的几何信息错误而在图像中产生模糊和伪影。我们提出了一种在线校准方法，即在样本台底部附着一层随机分散的微玻璃珠或钙颗粒。该方法基于样本台-标记层组件刚体运动的参数表示。标记层易于制作，且在校准过程中已被证明有效。