Guangdong Technology Research Center for Marine Algal Bioengineering, Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Longhua Innovation Institute for Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Mar Drugs. 2022 Sep 25;20(10):603. doi: 10.3390/md20100603.
Microalgae are promising microorganisms used to produce value-added products or to develop sustainable approaches for environmental remediation. The ATP-binding cassette proteins (ABCs) of have been characterized as indispensable transporters for CO concentrating mechanism, lipid biosynthesis, and heavy metal sequestration. However, few microalgal ABC proteins have been studied compared with higher plants or non-photosynthetic microorganisms. This study performed a genome-wide, evolutionary, and transcriptomic survey of ABC proteins (CrABCs). A total of 75 were identified and classed into eight ABC subfamilies, from ABCA to ABCI. We found that no whole or partial genome duplication events occurred in after the ancient endosymbiosis events, but gene duplications occurred in a small range of chromosomal regions, which forced ABC family expansion. Abundant light, abscisic acid, and jasmonic acid response -elements were mapped in the promoters, coinciding with the evolutionary history of hormone signaling in . The expression survey under light/dark rhythms revealed a close bond of with cell division and development. A broad study of CrABCs supported their expected roles in heavy metal detoxification, lipid metabolism, and environmental adaptation. Moreover, the evolutionary and expression survey predicted the functions of unknown CrABCs, which are elaborated in the text. Two half-size CrABCGs-CrABCG3 and CrABCG26-were described as plasma-membrane transporters that might participate in lipidic compound secretion. This study provides fundamental and exhaustive information about , which are indispensable for the functional elucidation of ABC proteins in microalgae.
微藻是一种很有前途的微生物，可用于生产增值产品或开发可持续的环境修复方法。 的 ATP 结合盒蛋白（ABC）已被描述为 CO 浓缩机制、脂类生物合成和重金属螯合所必需的转运蛋白。然而，与高等植物或非光合微生物相比，研究的微藻 ABC 蛋白很少。本研究对 的 ABC 蛋白（CrABCs）进行了全基因组、进化和转录组调查。共鉴定出 75 个 CrABCs，并将其分为 ABCA 到 ABCI 八个 ABC 亚家族。我们发现，在远古内共生事件之后， 中没有发生全基因组或部分基因组复制事件，但在小范围的染色体区域发生了基因复制，这迫使 ABC 家族扩张。在 的启动子中，映射了丰富的光、脱落酸和茉莉酸响应元件，这与 中激素信号转导的进化历史相符。在光/暗节律下的表达调查显示， 与细胞分裂和发育密切相关。对 CrABCs 的广泛研究支持了它们在重金属解毒、脂类代谢和环境适应中的预期作用。此外，进化和表达调查预测了未知 CrABCs 的功能，在正文中进行了详细阐述。两个半尺寸的 CrABCGs-CrABCG3 和 CrABCG26-被描述为质膜转运蛋白，可能参与脂类化合物的分泌。本研究提供了关于 的基本和详尽信息，这对于阐明微藻中 ABC 蛋白的功能是必不可少的。
