Medical Biotechnology, Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Bedford Park, Adelaide, SA 5042, Australia.
Key Centre for Polymers and Colloids, School of Chemistry, The University of Sydney, Sydney, NSW 2006, Australia.
Mar Drugs. 2022 Oct 21;20(10):655. doi: 10.3390/md20100655.
In this study, we have demonstrated a bioprocessing approach encompassing the exogenous addition of low-molecular-weight compounds to tune the fatty acid (FA) profile in a novel thraustochytrid strain to produce desirable FAs. Maximum lipid recovery (38%, dry wt. biomass) was obtained at 1% Tween 80 and 0.25 mg/L of Vitamin B12. The transesterified lipid showed palmitic acid (C16, 35.7% TFA), stearic acid (C18, 2.1% TFA), and oleic acid (C18:1, 18.7% TFA) as the main components of total FAs, which are mainly present in plant oils. Strikingly, D-limonene addition in the fermentation medium repressed the production of polyunsaturated fatty acid (PUFAs). Sulfur-polymerization-guided lipid separation revealed the presence of saturated (SFAs, 53% TFA) and monounsaturated fatty acids (MUFAs, 46.6% TFA) in thraustochytrid oil that mimics plant-oil-like FA profiles. This work is industrially valuable and advocates the use of sulfur polymerization for preparation of plant-like oils through tuneable thraustochytrid lipids.
在这项研究中，我们展示了一种生物处理方法，包括添加低分子量化合物来调节新型硫丝菌脂肪酸 (FA) 谱，以生产理想的 FA。在 1%吐温 80 和 0.25mg/L 维生素 B12 的条件下，脂质回收率达到最大值（干重生物质的 38%）。经酯交换的脂质显示出棕榈酸 (C16，35.7%TFA)、硬脂酸 (C18，2.1%TFA) 和油酸 (C18:1，18.7%TFA) 作为总 FA 的主要成分，这些 FA 主要存在于植物油中。引人注目的是，在发酵培养基中添加 D-苎烯会抑制多不饱和脂肪酸 (PUFA) 的产生。硫聚合引导的脂质分离显示，硫丝菌油中存在饱和脂肪酸 (SFAs，53%TFA) 和单不饱和脂肪酸 (MUFAs，46.6%TFA)，类似于植物油脂的 FA 谱。这项工作具有工业价值，提倡通过可调节的硫丝菌脂质来利用硫聚合制备植物油脂。
