Department of Ophthalmology, Joint Shantou International Eye Center, Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.
Photobiomodul Photomed Laser Surg. 2022 Oct;40(10):715-727. doi: 10.1089/photob.2022.0058.
To assess the inhibitory effects of intense pulsed light (IPL) on meibomian gland (MG) inflammation. IPL treatment is effective for dry eye disease (DED) caused by meibomian gland dysfunction (MGD). However, the anti-inflammatory and regeneration stimulating effects of IPL on MGD remain unclear. Moreover, studies on inflammatory metabolites in MG secretions are lacking. Six patients with DED were administered two IPL treatments. Ocular surface disease index (OSDI) questionnaires were used to assess DED, MGD signs, including degree of obstruction, secretion, and atrophy of the MG, tear film break-up time (TBUT) was assessed before and after treatments. To determine IPL treatment-induced changes in metabolites, liquid chromatography tandem mass spectrometry (LC-MS/MS) was used to analyze MG secretions. Data were gathered before the first treatment (time A) and 2 weeks after the second treatment (time B). Average OSDI score showed a significant decrease (time A and B measurements were 44.07 and 16.65, respectively). Besides, statistically significant differences were observed in MG signs before and after treatments: degree of obstruction improved and secretions became thinner. TBUT was significantly increased to the normal range. LC-MS/MS led to the identification of 53 differential metabolites: 23 were upregulated (e.g., estradiol, coenzyme Q, and azelaic acid) and 30 were downregulated (e.g., prostaglandins, 20-hydroxyeicosatetraenoic acid, and arachidonic acid). Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that most differential metabolites were involved in steroid hormone biosynthesis. Periorbital IPL treatment can improve chronic inflammation of the MG and promote its normal secretion. The steroid hormone biosynthetic pathways may be activated to participate in this anti-inflammatory effect.
评估强脉冲光 (IPL) 对睑板腺 (MG) 炎症的抑制作用。 IPL 治疗对睑板腺功能障碍 (MGD) 引起的干眼症 (DED) 有效。然而，IPL 对 MGD 的抗炎和再生刺激作用尚不清楚。此外，关于 MG 分泌物中炎症代谢物的研究还很少。 六例 DED 患者接受了两次 IPL 治疗。使用眼表疾病指数 (OSDI) 问卷评估 DED、MGD 体征，包括阻塞程度、分泌物和 MG 萎缩，在治疗前后评估泪膜破裂时间 (TBUT)。为了确定 IPL 治疗引起的代谢物变化，使用液相色谱串联质谱 (LC-MS/MS) 分析 MG 分泌物。数据在第一次治疗前 (时间 A) 和第二次治疗后 2 周 (时间 B) 收集。平均 OSDI 评分显著降低（时间 A 和 B 的测量值分别为 44.07 和 16.65）。此外，MG 体征在治疗前后有统计学差异：阻塞程度改善，分泌物变薄。TBUT 显著增加到正常范围。LC-MS/MS 鉴定出 53 种差异代谢物：23 种上调（如雌二醇、辅酶 Q 和壬二酸），30 种下调（如前列腺素、20-羟二十碳四烯酸和花生四烯酸）。京都基因与基因组百科全书途径富集分析显示，大多数差异代谢物参与甾体激素生物合成。眶周 IPL 治疗可改善 MG 的慢性炎症，促进其正常分泌。甾体激素生物合成途径可能被激活以参与这种抗炎作用。
