Ke Xing, Tian Yimei, He Dandan, Mu Pengqian, Wan Xuzhi, Zhang Lange, Jia Wei, Wang Qiao, Fan Yilei, Zhang Yu
Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Department of Criminal Science and Technology, Zhejiang Police College, Hangzhou 310053, China.
National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Toxics. 2022 Oct 18;10(10):619. doi: 10.3390/toxics10100619.
Synthetic cannabinoids, a class of psychoactive compounds, are controlled as new psychoactive substances (NPSs) identified by the early warning system (EWS) of the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA). At present, several new synthetic cannabinoids have appeared in the illegal drug market, including 4-methylnaphthalen-1-yl-(1-pentylindol-3-yl) methanone (JWH-122), methyl (1-(5-fluoropentyl)-1H-indazole-3-carbonyl)-L-valinate (5F-AMB), and methyl 2-(1-(4-fluorobenzyl)-1Hindazole-3-carboxamido)-3-methylbutanoate (AMB-FUBINACA). A convenient, rapid, and highly sensitive analytical method was developed to determine three synthetic cannabinoids in rat plasma and urine. The liquid chromatography tandem mass spectrometry (LC-MS/MS) method was optimized and validated to analyze the three synthetic cannabinoids in rat plasma and urine. The method identified intra-assay precision (1.3-9.0% and 2.8-6.7%), inter-assay precision (3.0-8.6% and 3.9-8.8%), limits of detection (0.003-0.004 ng/mL and 0.00125-0.002 ng/mL) and quantification (0.012-0.016 ng/mL and 0.003-0.005 ng/mL), recovery (95.4-106.8% and 92.0-106.8%) for rat plasma and urine, and the matrix effect (93.4-118.0%) for rat urine, and the correlation coefficients were above 0.99 in the linear range. The established LC-MS/MS method was successfully used to simultaneously detect the JWH-122 and 5F-AMB in rat plasma and JWH-122, 5F-AMB, and AMB-FUBINACA in rat urine. The present study provides methodological support for internal exposure assessment of three synthetic cannabinoids and promotes the quantitative analysis and technical supervision of synthetic cannabinoids.
合成大麻素是一类精神活性化合物，作为新精神活性物质（NPSs）受到欧洲毒品和药物成瘾监测中心（EMCDDA）早期预警系统（EWS）的管控。目前，几种新型合成大麻素已出现在非法毒品市场，包括4-甲基萘-1-基-（1-戊基吲哚-3-基）甲酮（JWH-122）、甲基（1-（5-氟戊基）-1H-吲唑-3-羰基）-L-缬氨酸酯（5F-AMB）和2-（1-（4-氟苄基）-1H-吲唑-3-甲酰胺基）-3-甲基丁酸甲酯（AMB-FUBINACA）。本研究建立了一种简便、快速且高灵敏的分析方法，用于测定大鼠血浆和尿液中的三种合成大麻素。对液相色谱串联质谱（LC-MS/MS）法进行了优化和验证，以分析大鼠血浆和尿液中的三种合成大麻素。该方法确定了大鼠血浆和尿液的批内精密度（1.3 - 9.0%和2.8 - 6.7%）、批间精密度（3.0 - 8.6%和3.9 - 8.8%）、检测限（0.003 - 0.004 ng/mL和0.00125 - 0.002 ng/mL）和定量限（0.012 - 0.016 ng/mL和0.003 - 0.005 ng/mL）、回收率（95.4 - 106.8%和92.0 - 106.8%）以及大鼠尿液的基质效应（93.4 - 118.0%），且线性范围内相关系数均高于0.99。所建立的LC-MS/MS方法成功用于同时检测大鼠血浆中的JWH-122和5F-AMB以及大鼠尿液中的JWH-122、5F-AMB和AMB-FUBINACA。本研究为三种合成大麻素的体内暴露评估提供了方法学支持，并促进了合成大麻素的定量分析和技术监管。
