Small Molecule Pharmaceutical Sciences, Genentech Inc., 1 DNA Way, South San Francisco, California 94080, United States.
Analytical Research and Development, AbbVie Inc., 1 North Waukegan Road, North Chicago, Illinois 60064, United States.
Anal Chem. 2022 Nov 22;94(46):16095-16102. doi: 10.1021/acs.analchem.2c03276. Epub 2022 Oct 26.
The advent of benchtop nuclear magnetic resonance (NMR) instrumentation has paved the way for the use of this technology away from traditional NMR facility settings. Still, a wider adoption of benchtop NMR systems for routine identification testing has been hampered by inherent instrumental limitations (including low sensitivity and reduced signal dispersion) and workflow automation challenges. The present study summarizes the results of a cross-company collaboration aiming at the development of rapid, automated identification tests for incoming materials in liquid form intended for pharmaceutical manufacturing. Potential scenarios that analysts may encounter during the development of identification tests using benchtop NMR instrumentation are described, and suitable strategies for data collection and analysis are discussed. Challenges and opportunities for benchtop NMR implementation are illustrated using common organic solvents and laboratory reagents in a neat form, for which reference NMR data are provided.
台式核磁共振（NMR）仪器的出现为该技术在传统 NMR 设备设置之外的应用铺平了道路。然而，由于仪器固有的限制（包括低灵敏度和信号分散减少）以及工作流程自动化的挑战，台式 NMR 系统在常规鉴定测试中的更广泛应用受到了阻碍。本研究总结了一项跨公司合作的结果，旨在开发用于制药生产的来料的快速、自动化的液体形式的鉴定测试。描述了分析人员在使用台式 NMR 仪器进行鉴定测试的开发过程中可能遇到的潜在情况，并讨论了适合的数据收集和分析策略。使用常见的有机溶剂和实验室试剂以纯物质形式展示了台式 NMR 实施的挑战和机遇，并提供了参考 NMR 数据。
