Steffen R, Ballmer F T, Luder P J, Mettler D, Barbier P A
Res Exp Med (Berl). 1987;187(4):265-74. doi: 10.1007/BF01852052.
Extracorporeal isolated pig liver perfusion was performed in three groups of five piglets each to assess the influence of different blood primes on liver function. All prime volumes were hemodiluted to a hematocrit of 0.3-0.35 and adjusted to a pH of 7.35-7.45 with sodium bicarbonate at a temperature of 38 degrees C. Fresh slaughterhouse blood was collected, mechanically filtered and used in Group A, whereas analogously collected blood was biologically filtered by passage through the organism of the piglet in Group B. In Group C, the priming blood consisted of autologous blood gained during hepatectomy by a special technique. A high potassium concentration (10.4 +/- 0.94 mmol/l) in Group A and impurities of the slaughterhouse blood in both Groups A and B led to rapid edema formation and an increase in vascular resistance of the perfused liver, and a prolonged perfusion was not achieved. In the third group, however, liver perfusion over a period of 5 h without any deterioration of liver function was possible. Beside liver function tests, oxygen consumption, the macroscopic aspect and bile flow of the liver seemed to be the most reliable parameters in assessing the function of the isolated perfused pig liver. Our technique to gain autologous blood can be recommended not only for extracorporeal liver perfusion but also for any other extracorporeal isolated organ perfusion.
对三组仔猪（每组五只）进行体外孤立猪肝灌注，以评估不同血液预充液对肝功能的影响。所有预充液体积均进行血液稀释，使血细胞比容达到0.3 - 0.35，并在38℃用碳酸氢钠将pH值调整至7.35 - 7.45。收集新鲜屠宰场血液，机械过滤后用于A组，而B组类似收集的血液则通过仔猪机体进行生物过滤。C组的预充血液由通过特殊技术在肝切除术中获得的自体血液组成。A组的高钾浓度（10.4±0.94 mmol/l）以及A组和B组屠宰场血液中的杂质导致灌注肝脏迅速形成水肿并增加血管阻力，未能实现长时间灌注。然而，在第三组中，肝脏灌注5小时且肝功能无任何恶化是可行的。除肝功能测试外，肝脏的氧消耗、宏观外观和胆汁流量似乎是评估孤立灌注猪肝功能最可靠的参数。我们获取自体血液的技术不仅可推荐用于体外肝脏灌注，也可用于任何其他体外孤立器官灌注。
