通过综合物理化学方法揭示的灭活蜱传脑炎病毒颗粒的大小分布

Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach.

作者信息

Moiseenko Andrey V, Bagrov Dmitry V, Vorovitch Mikhail F, Uvarova Victoria I, Veselov Maxim M, Kashchenko Anastasia V, Ivanova Alla L, Osolodkin Dmitry I, Egorov Alexey M, Ishmukhametov Aydar A, Shaitan Konstantin V, Sokolova Olga S

机构信息

Department of Biology, Lomonosov Moscow State University, Moscow 119234, Russia.

FSASI "Chumakov FSC R&D IBP RAS" (Institute of Poliomyelitis), Moscow 108819, Russia.

出版信息

Biomedicines. 2022 Oct 4;10(10):2478. doi: 10.3390/biomedicines10102478.

DOI:10.3390/biomedicines10102478

PMID:36289740

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9598892/

Abstract

Tick-borne encephalitis virus (TBEV) is an enveloped RNA virus, a member of the genus (family ). Here, we provide a detailed analysis of the size and structure of the inactivated TBEV vaccine strain Sofjin-Chumakov. Four analytical methods were used to analyze individual TBEV particles-negative staining TEM, cryo-EM, atomic force microscopy (AFM), and nanoparticle tracking analysis (NTA). All methods confirmed that the particles were monodisperse and that their mean size was ~50 nm. Cryo-EM data allowed us to obtain a 3D electron density model of the virus with clearly distinguishable E protein molecules. STEM-EELS analysis detected phosphorus in the particles, which was interpreted as an indicator of RNA presence. Altogether, the described analytical procedures can be valuable for the characterization of inactivated vaccine virus samples.

摘要

蜱传脑炎病毒（TBEV）是一种有包膜的RNA病毒，属于（科）属的成员。在此，我们对灭活的TBEV疫苗株索非金-丘马科夫的大小和结构进行了详细分析。使用了四种分析方法来分析单个TBEV颗粒——负染色透射电子显微镜（TEM）、冷冻电镜（cryo-EM）、原子力显微镜（AFM）和纳米颗粒跟踪分析（NTA）。所有方法均证实颗粒呈单分散状态，其平均大小约为50纳米。冷冻电镜数据使我们能够获得病毒的三维电子密度模型，其中E蛋白分子清晰可辨。扫描透射电子显微镜-电子能量损失谱（STEM-EELS）分析在颗粒中检测到了磷，这被解释为RNA存在的指标。总之，所描述的分析程序对于灭活疫苗病毒样本的表征可能具有重要价值。