Elsherbini Mohammed, Koga Kaori, Maki Eiko, Kumasawa Keiichi, Satake Erina, Taguchi Ayumi, Makabe Tomoko, Takeuchi Arisa, Izumi Gentaro, Takamura Masashi, Harada Miyuki, Hirata Tetsuya, Hirota Yasushi, Wada-Hiraike Osamu, Osuga Yutaka
Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo, Tokyo 113-8654, Japan.
Department of Obstetrics and Gynecology, Faculty of Medicine, Saitama Medical University, Saitama 350-0495, Japan.
Biomedicines. 2022 Oct 19;10(10):2627. doi: 10.3390/biomedicines10102627.
The purpose of this study was to establish a new mouse model of endometriosis that mimics real-world women's health problems, in which women continue to be affected by endometriosis long before they wish to become pregnant, and to evaluate the impact of "chronic exposure to endometriosis" on perinatal outcome. Endometriosis was established by the intraperitoneal injection of homologous minced mouse uteri. Vehicle was injected for the control. Mating was initiated either 1 or 43 days after disease establishment (Young or Aged studies, respectively). Mice were sacrificed on 18 dpc. The number pups and resorptions were counted and pups' body weights (BW) were measured, and the endometriosis lesion was identified and weighted. In the Young study, the number of resorptions and BW were comparable between the groups. In the Aged study, the number of resorptions was significantly higher and BW was significantly lower in endometriosis than that in control. The total weight of endometriosis lesion per dam was significantly lower in the Aged compared to the Young endometriosis group; however, not a single mouse was found to have any lesions at all. These results suggest that in addition to the presence of endometriosis per se, "chronic exposure to endometriosis" prior to pregnancy affect perinatal outcomes.
本研究的目的是建立一种新的子宫内膜异位症小鼠模型，该模型可模拟现实世界中女性的健康问题，即女性在希望怀孕之前很长一段时间就持续受到子宫内膜异位症的影响，并评估“长期暴露于子宫内膜异位症”对围产期结局的影响。通过腹腔注射同源的切碎小鼠子宫来建立子宫内膜异位症模型。注射赋形剂作为对照。在疾病建立后1天或43天开始交配（分别为年轻组或老年组研究）。在妊娠第18天处死小鼠。计算幼崽数量和吸收胎数量，测量幼崽体重（BW），并识别和称重子宫内膜异位症病变。在年轻组研究中，各组之间的吸收胎数量和BW相当。在老年组研究中，子宫内膜异位症组的吸收胎数量显著更高，BW显著更低。与年轻子宫内膜异位症组相比，老年组每只母鼠的子宫内膜异位症病变总重量显著更低；然而，未发现有任何一只小鼠完全没有病变。这些结果表明，除了子宫内膜异位症本身的存在外，怀孕前“长期暴露于子宫内膜异位症”会影响围产期结局。
