源自实验室群体的新细胞系以及[具体名称未给出]的载体不携带锥蝽病毒。
New Cell Lines Derived from Laboratory Colony and , Vectors of , Do Not Harbour Triatoma Virus.
作者信息
Penrice-Randal Rebekah, Hartley Catherine, Beliavskaia Alexandra, Dong Xiaofeng, Brandner-Garrod Luke, Whitten Miranda, Bell-Sakyi Lesley
机构信息
Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, 146 Brownlow Hill, Liverpool L3 5RF, UK.
Department of Infection Biology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK.
出版信息
Insects. 2022 Oct 5;13(10):906. doi: 10.3390/insects13100906.
Triatomine bugs of the genera and are vectors of Chagas disease, a neglected tropical disease of humans in South America caused by . Triatoma virus (TrV), a natural pathogen of , has been proposed as a possible tool for the bio-control of triatomine bugs, but research into this virus has been hampered by a lack of suitable host cells for propagation. Here we report establishment and partial characterisation of continuous cell lines from embryos of (TIE/LULS54) and (RPE/LULS53 and RPE/LULS57). RNAseq screening by a sequence-independent, single primer amplification approach confirmed the absence of TrV and other RNA viruses known to infect , indicating that these new cell lines could be used for propagation of TrV.
属和属的锥蝽是恰加斯病的传播媒介，恰加斯病是南美洲一种被忽视的人类热带疾病，由[病原体名称缺失]引起。锥蝽病毒（TrV）是[锥蝽属名称缺失]的一种天然病原体，已被提议作为一种可能的锥蝽生物防治工具，但对这种病毒的研究因缺乏适合其繁殖的宿主细胞而受到阻碍。在这里，我们报告了从[锥蝽属名称缺失]（TIE/LULS54）和[锥蝽属名称缺失]（RPE/LULS53和RPE/LULS57）胚胎中建立连续细胞系并对其进行部分特性鉴定。通过一种不依赖序列的单引物扩增方法进行的RNAseq筛选证实不存在TrV和其他已知感染[锥蝽属名称缺失]的RNA病毒，这表明这些新的细胞系可用于TrV的繁殖。
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