Laboratory for Molecular Structural Dynamics, Theory Department, National Institute of Chemistry, 1000 Ljubljana, Slovenia.
Institute of Anatomy, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
Int J Mol Sci. 2022 Oct 18;23(20):12498. doi: 10.3390/ijms232012498.
Age, obesity, and diabetes mellitus are pathophysiologically interconnected factors that significantly contribute to the global burden of non-communicable diseases. These metabolic conditions are associated with impaired insulin function, which disrupts the metabolism of carbohydrates, lipids, and proteins and can lead to structural and functional changes in skeletal muscle. Therefore, the alterations in the macromolecular composition of skeletal muscle may provide an indication of the underlying mechanisms of insulin-related disorders. The aim of this study was to investigate the potential of Fourier transform infrared (FTIR) spectroscopy to reveal the changes in macromolecular composition in weight-bearing and non-weight-bearing muscles of old, obese, insulin-resistant, and young streptozotocin (STZ)-induced diabetic mice. The efficiency of FTIR spectroscopy was evaluated by comparison with the results of gold-standard histochemical techniques. The differences in biomolecular phenotypes and the alterations in muscle composition in relation to their functional properties observed from FTIR spectra suggest that FTIR spectroscopy can detect most of the changes observed in muscle tissue by histochemical analyses and more. Therefore, it could be used as an effective alternative because it allows for the complete characterization of macromolecular composition in a single, relatively simple experiment, avoiding some obvious drawbacks of histochemical methods.
年龄、肥胖和糖尿病是病理生理学上相互关联的因素，它们极大地促成了非传染性疾病的全球负担。这些代谢状况与胰岛素功能受损有关，这会破坏碳水化合物、脂肪和蛋白质的代谢，并导致骨骼肌的结构和功能变化。因此，骨骼肌的大分子组成的改变可能为与胰岛素相关的疾病的潜在机制提供指示。本研究的目的是探讨傅里叶变换红外（FTIR）光谱学揭示负重和非负重肌肉中大分子组成变化的潜力，这些肌肉来自年老、肥胖、胰岛素抵抗和年轻链脲佐菌素（STZ）诱导的糖尿病小鼠。FTIR 光谱学的效率通过与黄金标准组织化学技术的结果进行比较来评估。从 FTIR 光谱中观察到生物分子表型的差异以及与肌肉功能特性相关的肌肉组成的改变表明，FTIR 光谱学可以检测到组织化学分析中观察到的大多数肌肉组织变化，甚至更多。因此，它可以用作有效的替代方法，因为它允许在单次相对简单的实验中对大分子组成进行完整的特征描述，从而避免了组织化学方法的一些明显缺点。
