Scientific Observing and Experimental Station of Crop Pests in Guiyang, Ministry of Agricultural and Rural Affairs, Institute of Entomology, Guizhou University, Guiyang 550025, China.
College of Life Science, Guizhou University, Guiyang 550025, China.
Int J Mol Sci. 2022 Oct 19;23(20):12535. doi: 10.3390/ijms232012535.
Dallas is used as a traditional Chinese medicine as well as an edible insect. Although its anti-tumor effects have been observed, the anti-tumor active component(s) in the hemolymph of remains unknown. In this study, a combination usage of ultrafiltration, gel filtration chromatography, FPLC and RP-HPLC to separate and purify active peptides was performed based on the proliferation of the human gastric cancer SGC-7901 cell line treated with candidates. One peptide (MW = 2853.3 Da) was isolated from the hemolymph of A total of 24 amino acid residues were continuously determined for the active peptide: N'-ECGYCAEKGIRCDDIHCCTGLKKK-C'. In conclusion, a peptide that can inhibit the proliferation of gastric cancer SGC-7901 cells in the hemolymph of was purified in this study, which is homologous to members of the spider toxin protein family. These results should facilitate further works for this peptide, such as the cloning of genes, expression in vitro by prokaryotic or eukaryotic systems, more specific tests of anti-tumor activity, and so on.
作为一种传统中药和食用昆虫，被广泛应用。虽然其具有抗肿瘤作用，但血液中的抗肿瘤活性成分仍不清楚。本研究采用超滤、凝胶过滤色谱、FPLC 和反相高效液相色谱（RP-HPLC）相结合的方法，根据候选物对人胃癌 SGC-7901 细胞系增殖的影响，对活性肽进行分离和纯化。从血液中分离得到一种肽（MW = 2853.3 Da）。该活性肽的 24 个连续氨基酸残基序列为：N'-ECGYCAEKGIRCDDIHCCTGLKKK-C'。总之，本研究从血液中纯化出一种能抑制胃癌 SGC-7901 细胞增殖的肽，该肽与蜘蛛毒素蛋白家族成员同源。这些结果将有助于该肽的进一步研究，如基因克隆、原核或真核系统的体外表达、更特异的抗肿瘤活性测试等。
