State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Int J Mol Sci. 2022 Oct 19;23(20):12557. doi: 10.3390/ijms232012557.
The cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C (AGC) perform various functions in plants, involving growth, immunity, apoptosis and stress response. gene family is well described in , however, limited information is provided about genes in rice, an important cereal crop. This research studied the gene family in the AA genome species: ssp. , ssp. , , , , , , and were searched and classified into six subfamilies, and it was found that these species have similar numbers of members. The analysis of gene duplication and selection pressure indicated that the gene family expanded mainly by segmental or whole genome duplication (WGD), with purifying selection during the long evolutionary period. RNA-seq analysis revealed that of subfamily V were specifically highly expressed in leaves, and the expression patterns of these genes were compared with that of photosynthesis-related genes using qRT-PCR, discovered that , , and might participate in photosynthesis. These results provide an informative perspective for exploring the evolutionary of gene family and its practical application in rice.
依赖 cAMP 的蛋白激酶 A、依赖 cGMP 的蛋白激酶 G 和依赖磷脂的蛋白激酶 C（AGC）在植物中发挥着各种功能，涉及生长、免疫、细胞凋亡和应激反应。在 中，该基因家族得到了很好的描述，然而，关于水稻中 基因的信息有限，水稻是一种重要的谷类作物。本研究对 AA 基因组物种中的 基因家族进行了研究： ssp. 、 ssp. 、 、 、 、 和 。对这些物种进行了搜索和分类，发现它们的成员数量相似。基因复制和选择压力分析表明，该基因家族主要通过片段或全基因组复制（WGD）扩张，在漫长的进化过程中受到了纯化选择。RNA-seq 分析表明，V 亚家族的 个成员在叶片中特异性高表达，并用 qRT-PCR 比较了这些基因与光合作用相关基因的表达模式，发现 、 和 可能参与光合作用。这些结果为探索 基因家族的进化及其在水稻中的实际应用提供了有价值的视角。
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