Hu Yanhong, He Zhihua, Kang Yebin, Ye Wenwu, Cui Linkai
College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471000, China.
Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China.
J Fungi (Basel). 2022 Sep 22;8(10):998. doi: 10.3390/jof8100998.
is a destructive soybean pathogen that orchestrates various secreted proteins (effectors) to modulate plant immunity and facilitate infection. Although a number of effectors have been identified and functionally studied in , the way these molecules are regulated is marginally known. In this study, we performed a weighted gene correlation network analysis (WGCNA) based on digital RNA-seq, which enabled the identification of a transcription factor (PsCZF3) in . This transcription factor is a CH-type zinc finger protein that regulates the transcription of 35 RxLR effectors during the early infection stage. Phylogenetic analysis revealed that PsCZF3 is a highly conserved protein across oomycetes, suggesting that this regulation mechanism may broadly exist in oomycete species. In addition, by building a subnetwork of and correlated genes, we also found that PsCZF3 contributed to the transcriptional regulation of carbohydrate-active enzymes. Our findings suggest that the activation of facilitates infection by up-regulating RxLR effectors and carbohydrate-active enzymes.
是一种具有破坏性的大豆病原体，它调控多种分泌蛋白（效应子）来调节植物免疫并促进感染。尽管已经在中鉴定出许多效应子并对其进行了功能研究，但这些分子的调控方式却鲜为人知。在本研究中，我们基于数字RNA测序进行了加权基因共表达网络分析（WGCNA），从而在中鉴定出一种转录因子（PsCZF3）。该转录因子是一种CH型锌指蛋白，在感染早期阶段调控35种RxLR效应子的转录。系统发育分析表明，PsCZF3是卵菌中高度保守的蛋白，这表明这种调控机制可能广泛存在于卵菌物种中。此外，通过构建与相关基因的子网，我们还发现PsCZF3有助于碳水化合物活性酶的转录调控。我们的研究结果表明，的激活通过上调RxLR效应子和碳水化合物活性酶来促进感染。
