Dale Jenna C M, Newman Jonathan A
Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada.
Department of Biology, Wilfrid Laurier University, Waterloo, ON N2L 3C5, Canada.
J Fungi (Basel). 2022 Sep 28;8(10):1026. doi: 10.3390/jof8101026.
Tall fescue () is a cool-season grass which is commonly infected with the fungal endophyte . Although the relationship between tall fescue and is well-studied, less is known about its broader fungal communities. We used next-generation sequencing of the ITS2 region to describe the complete foliar fungal microbiomes in a set of field-grown tall fescue plants over two years, and whether these fungal communities were affected by the presence of . We used the Georgia 5 cultivar of tall fescue, grown in the field for six years prior to sampling. Plants were either uninfected with , or they were infected with one of two strains: The common toxic strain or the AR542 strain (sold commerically as MaxQ). We observed 3487 amplicon sequence variants (ASVs) across all plants and identified 43 ASVs which may make up a potential core microbiome. Fungal communities did not differ strongly between treatments, but did show a great deal of variation between the two years. Plant fitness also changed over time but was not influenced by infection.
高羊茅（）是一种冷季型草，通常会被真菌内生菌感染。虽然高羊茅与之间的关系已得到充分研究，但对其更广泛的真菌群落了解较少。我们使用第二代测序技术对ITS2区域进行测序，以描述一组田间种植的高羊茅植物在两年内完整的叶片真菌微生物群落，以及这些真菌群落是否受到的存在的影响。我们使用了高羊茅的Georgia 5品种，在采样前已在田间种植了六年。植物要么未被感染，要么被两种菌株之一感染：常见的有毒菌株或AR542菌株（作为MaxQ商业销售）。我们在所有植物中观察到3487个扩增子序列变体（ASV），并鉴定出43个可能构成潜在核心微生物群的ASV。不同处理之间的真菌群落差异不大，但在两年之间确实表现出很大的变化。植物适应性也随时间变化，但不受感染的影响。
