Wu Yi-Hua, Chen De-Yun, Wang Xin-Ju, Li Neng-Qi, Gao Jiang-Yun
Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming 650500, China.
J Fungi (Basel). 2022 Sep 29;8(10):1036. doi: 10.3390/jof8101036.
Using orchid mycorrhizal fungi (OMFs) to facilitate orchid proliferation is considered an effective method of orchid conservation. Based on the success of using in situ seedling baiting to obtain plant growth-promoting fungi in our previous study, in this study, we developed the method of using ex situ seedling baiting to capture seedling-associated fungi from . We collected substrates (e.g., litters, barks and mosses) from six original habitats of in different geographical locations in China, and then, transplanted in vitro-produced seedlings of into the substrates. After cultivation for 75 days, it was obvious that fungi colonized the seedling roots and formed large numbers of pelotons in all six groups. From these seedling roots, a total of 251 fungal strains, which were divided into 16 OMF and 11 non-OMF species, were successfully isolated. The 16 OMFs included 13 and 3 Serendipitaceae species. The fungal species isolated from the different groups (original habitat sources) were not identical, but the dominant OMFs with high isolation frequencies (more than 10 times) were commonly isolated from more than four original sources. Among the 11 non-OMFs, TP-18 and sp. TP-26 were the dominant endophytes. is a common endophyte associated with many orchid species, including . The results suggest that ex situ seedling baiting is an easy and efficient approach to obtaining seedling-associated fungi for this species and could be performed for other over-collected species, especially orchids for which wild plants have disappeared in the field but their original habitats are known. This approach has great potential for application in OMF studies in the future.
利用兰花菌根真菌（OMF）促进兰花繁殖被认为是一种有效的兰花保护方法。基于我们之前研究中利用原位幼苗诱捕法成功获取促进植物生长真菌的经验，在本研究中，我们开发了利用异位幼苗诱捕法从……捕获与幼苗相关真菌的方法。我们从中国不同地理位置的6个……原生栖息地收集了基质（如枯枝落叶、树皮和苔藓），然后将体外培养的……幼苗移植到这些基质中。培养75天后，很明显真菌在所有6组幼苗根中定殖并形成了大量菌丝团。从这些幼苗根中，共成功分离出251株真菌菌株，分为16种OMF和11种非OMF物种。16种OMF包括13种……和3种丝核菌科物种。从不同组（原生栖息地来源）分离出的真菌物种并不相同，但分离频率高（超过10次）的优势OMF通常从4个以上的原生来源中分离得到。在11种非OMF中，……TP - 18和……sp. TP - 26是优势内生菌。……是一种与许多兰花物种相关的常见内生菌，包括……。结果表明，异位幼苗诱捕法是一种获取该物种与幼苗相关真菌的简便有效方法，可用于其他过度采集的物种，特别是那些野生植物在野外已消失但其原生栖息地已知的兰花。这种方法在未来的OMF研究中具有很大的应用潜力。
