Department of the Joint Laboratory of Biological Psychiatry, Mental Health Center of Shantou University, Shantou 515000, China.
Department of Medical Examination Center, Mental Health Center of Shantou University, Shantou 515000, China.
Nutrients. 2022 Oct 14;14(20):4302. doi: 10.3390/nu14204302.
Shortening is mainly derived from the partial hydrogenation of palm oil and widely used in fast food. Food processed with shortening contains high levels of industrial trans fatty acids. Studies have shown that there is a correlation between industrial trans fatty acids, obesity, and depression. However, the regulatory effect of neuronal nitric oxide synthase (nNOS) on depression in obese patients is still unknown. The purpose of this study was to explore mood changes in obese mice fed a high shortening diet, and to determine the regulatory effect of nNOS on depressive-like behaviors in obese mice. We used a high shortening diet-induced obesity mouse model to systematically assess the metabolic response, behavioral changes, prefrontal and hippocampal nNOS protein levels, and the effect of nNOS inhibitors (7-nitroindole) on depression-like behavior in obese mice. Interestingly, obese mice on a 9-week high-shortening diet developed short-term spatial working memory impairment and anxiety-like behavior, and obesity may be a risk factor for cognitive impairment and mood disorders. In animals fed a high shortening diet for 12 weeks, obese mice developed depression-like behavior and had significantly elevated levels of nNOS protein expression in the hippocampus and prefrontal lobe. Administration of the nNOS inhibitor 7-nitroindole could improve depression-like behaviors in obese mice, further suggesting that inhibition of nNOS is helpful for depression associated with obesity.
缩短是主要来源于部分氢化棕榈油，并广泛应用于快餐。用缩短加工的食品含有高水平的工业反式脂肪酸。研究表明，工业反式脂肪酸与肥胖和抑郁症之间存在关联。然而，神经元型一氧化氮合酶（nNOS）对肥胖患者抑郁的调节作用尚不清楚。本研究旨在探讨肥胖小鼠在喂食高缩短饮食后的情绪变化，并确定 nNOS 对肥胖小鼠抑郁样行为的调节作用。我们使用高缩短饮食诱导的肥胖小鼠模型来系统评估代谢反应、行为变化、前额叶和海马 nNOS 蛋白水平，以及 nNOS 抑制剂（7-硝基吲唑）对肥胖小鼠抑郁样行为的影响。有趣的是，肥胖小鼠在 9 周的高缩短饮食后出现短期空间工作记忆障碍和焦虑样行为，肥胖可能是认知障碍和情绪障碍的一个危险因素。在 12 周喂食高缩短饮食的动物中，肥胖小鼠出现抑郁样行为，并且在海马和前额叶中 nNOS 蛋白表达显著升高。给予 nNOS 抑制剂 7-硝基吲唑可以改善肥胖小鼠的抑郁样行为，这进一步表明抑制 nNOS 有助于肥胖相关的抑郁。
