Joint Biobank, Office of Human Research, Taipei Medical University, Taipei 110301, Taiwan.
Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110301, Taiwan.
Nutrients. 2022 Oct 19;14(20):4388. doi: 10.3390/nu14204388.
Preclinical studies have demonstrated that intrauterine growth retardation (IUGR) is associated with reduced lung development during the neonatal period and infancy. Uteroplacental insufficiency (UPI), affecting approximately 10% of human pregnancies, is the most common cause of IUGR. This study investigated the effects of UPI on lung development and the intestinal microbiota and correlations in newborn rats with IUGR, using bilateral uterine artery ligation to induce UPI. Maternal fecal samples were collected on postnatal day 0. On postnatal days 0 and 7, lung and intestinal microbiota samples were collected from the left lung and the lower gastrointestinal tract. The right lung was harvested for histological assessment and Western blot analysis. Results showed that UPI through bilateral uterine artery ligation did not alter the maternal gut microbiota. IUGR impaired lung development and angiogenesis in newborn rats. Moreover, on postnatal day 0, the presence of and in the lungs and and in the gastrointestinal tract was negatively correlated with lung development. in the lungs and and in the gastrointestinal tract were negatively correlated with lung development on day 7. UPI may have regulated lung development and angiogenesis through the modulation of the newborn rats' intestinal and lung microbiota.
临床前研究表明，宫内生长迟缓（IUGR）与新生儿期和婴儿期肺发育不良有关。影响约 10%人类妊娠的胎盘功能不全（UPI）是 IUGR 的最常见原因。本研究通过双侧子宫动脉结扎诱导 UPI，探讨了 UPI 对宫内生长受限新生大鼠肺发育和肠道微生物群及其相关性的影响。在产后第 0 天收集母体粪便样本。在产后第 0 天和第 7 天，从左肺和下消化道采集肺和肠道微生物群样本。采集右肺进行组织学评估和 Western blot 分析。结果表明，通过双侧子宫动脉结扎的 UPI 不会改变母体肠道微生物群。IUGR 会损害新生大鼠的肺发育和血管生成。此外，在产后第 0 天，肺部的 和 和胃肠道中的 与肺发育呈负相关。第 7 天，肺部的 和胃肠道中的 和 与肺发育呈负相关。UPI 可能通过调节新生大鼠的肠道和肺部微生物群来调节肺发育和血管生成。
