Pongprayoon Wasinee, Panya Atikorn, Jaresitthikunchai Janthima, Phaonakrop Narumon, Roytrakul Sittiruk
Department of Biology, Faculty of Science, Burapha University, 169 Longhaad Bangsaen Rd, Saensook, Mueang, Chonburi 20131, Thailand.
Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phaholyothin Rd., Klong Luang, Pathum Thani 12120, Thailand.
Plants (Basel). 2022 Oct 15;11(20):2729. doi: 10.3390/plants11202729.
This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.
本研究旨在鉴定梁普拉特维123（LPT123）和考道克马里105（KDML105）这两个干旱敏感型水稻品种中新型的壳聚糖（CTS）响应磷蛋白以及CTS响应的差异。水稻种子在萌发前浸泡于CTS溶液中，在渗透胁迫前对2周龄和4周龄的水稻幼苗喷施CTS，共分为以下四组：（1）用蒸馏水处理的幼苗；（2）用CTS处理的幼苗；（3）用蒸馏水预处理并遭受渗透胁迫的幼苗；（4）用CTS预处理并遭受渗透胁迫的幼苗。在胰蛋白酶消化和LC-MS分析之前，使用固定化金属亲和色谱（IMAC）富集叶片组织中的磷蛋白。磷蛋白谱分析分别鉴定出LPT123和KDML105幼苗中代表1052个和1040个独特磷蛋白的4721个磷蛋白。在渗透胁迫前进行CTS预处理后，发现了22种差异表达蛋白，其中分别在LPT123和KDML105中鉴定出10种和12种。这些蛋白通常参与信号传导、转运、蛋白质折叠、蛋白质降解和代谢。本研究为理解在暴露于渗透胁迫之前用CTS预处理的水稻幼苗的信号转导机制提供了丰富的数据。
