Deng Yahui, Chen Quanjia, Qu Yanying
Engineering Research Centre of Cotton, Ministry of Education, College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi 830052, China.
Plants (Basel). 2022 Oct 18;11(20):2758. doi: 10.3390/plants11202758.
Protein palmitoylation is an ability of the frame of the cell marker protein is one of the most notable reversible changes after translation. However, studies on protein palmitoylation in cotton have not yet been performed. In our current research, the PAT gene family was systematically identified and bioinformatically analyzed in , , and , and 211 PAT genes were authenticated and classified into six subfamilies. Sixty-nine PAT genes were identified in upland cotton, mainly at the ends of its the 26 chromosomes of upland cotton. The majority of these genes are located in the nucleus of the plant. Gene structure analysis revealed that each member encodes a protein that which contains at least one DHHC structural domain. Cis-acting element analysis indicated that GhPATs genes are mainly involved in hormone production, light response and stress response. Gene expression pattern analysis indicated that most GhPATs genes were differentially expressed upon induction by pathogenic bacteria, drought, salt, hot and cold stresses, and some GhPATs could be induced by multiple abiotic stresses simultaneously. GhPATs genes showed different expression patterns in tissue-specific assays and were found to be preferentially expressed in roots, followed by expression in stems and leaves. Virus-induced gene silencing (VIGS) experiments showed that cotton was significantly less resistant to when was silenced. We conclude that the gene, which mediates S-palmitoylation acetylation, may be involved in the regulation of upland cotton resistance to (VW). Overall, this work has provided a fundamental framework for understanding the latent capabilities of GhPATs and a solid foundation for molecular breeding and plant pathogen resistance in cotton.
蛋白质棕榈酰化是细胞标记蛋白框架的一种能力，是翻译后最显著的可逆变化之一。然而，尚未对棉花中的蛋白质棕榈酰化进行研究。在我们目前的研究中，对陆地棉、海岛棉、亚洲棉和草棉中的PAT基因家族进行了系统鉴定和生物信息学分析，鉴定出211个PAT基因并将其分为六个亚家族。在陆地棉中鉴定出69个PAT基因，主要位于陆地棉26条染色体的末端。这些基因大多数位于植物细胞核中。基因结构分析表明，每个成员编码的蛋白质至少包含一个DHHC结构域。顺式作用元件分析表明，GhPATs基因主要参与激素产生、光反应和应激反应。基因表达模式分析表明，大多数GhPATs基因在病原菌、干旱、盐、热和冷胁迫诱导下差异表达，一些GhPATs可同时被多种非生物胁迫诱导。GhPATs基因在组织特异性分析中表现出不同的表达模式，且在根中优先表达，其次是在茎和叶中表达。病毒诱导基因沉默（VIGS）实验表明，当GhPAT基因沉默时，棉花对黄萎病的抗性显著降低。我们得出结论，介导S-棕榈酰化乙酰化的GhPAT基因可能参与陆地棉对黄萎病抗性的调控。总体而言，这项工作为理解GhPATs的潜在能力提供了基本框架，并为棉花分子育种和植物病原菌抗性奠定了坚实基础。
