Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, Brazil.
Laboratory of Molecular Virology, Oswaldo Cruz Foundation of Rondônia-FIOCRUZ/RO, Porto Velho 76812-245, Brazil.
Viruses. 2022 Sep 22;14(10):2100. doi: 10.3390/v14102100.
The emergence of clinically relevant mutations in the hepatitis B virus (HBV) genome has been a matter of great debate because of the possibility of escape from the host's immune system, the potential to cause more severe progression of liver diseases and the emergence of treatment-resistant variants. Here we characterized the circulating variants of HBV in Rondônia State, in the north of Brazil. Serum samples of 62 chronic HBV carriers were subjected to PCR assays and clinical data were collected. Mutations and genotypes were characterized through direct sequencing. The findings show the presence of subgenotypes A1 (54.83%, 34/62), D3 (16.13%, 10/62), F2 (16.13%, 10/62), A2 (4.84%, 3/62), D2 (3.23%, 2/62), D1 (1.61%, 1/62), D4 (1.61%, 1/62) and F4 (1.61%, 1/62). Deletions in the pre-S2 region were found in 13.79% (8/58) of the samples, mutations in the S gene in 59.68% (37/62) and RT mutations in 48.39% (30/62). We found a variable genotypic distribution in different locations and important mutations related to immune escape and drug resistance in Western Amazonia, which contributed to genetic surveillance and provided important information to help control the disease.
乙型肝炎病毒 (HBV) 基因组中临床相关突变的出现一直是一个争论的焦点，因为这些突变有可能逃避宿主的免疫系统，导致肝脏疾病更严重的进展，并出现治疗耐药的变异体。在这里，我们对巴西北部朗多尼亚州的 HBV 循环变异体进行了特征描述。对 62 名慢性 HBV 携带者的血清样本进行了 PCR 检测，并收集了临床数据。通过直接测序对突变和基因型进行了特征描述。研究结果表明存在亚基因型 A1（54.83%，34/62）、D3（16.13%，10/62）、F2（16.13%，10/62）、A2（4.84%，3/62）、D2（3.23%，2/62）、D1（1.61%，1/62）、D4（1.61%，1/62）和 F4（1.61%，1/62）。在 58 个样本中有 13.79%（8/58）存在前 S2 区缺失，S 基因中有 59.68%（37/62）发生突变，逆转录酶区有 48.39%（30/62）发生突变。我们发现，在亚马逊西部地区不同地区存在不同的基因型分布，并且存在与免疫逃避和耐药性相关的重要突变，这有助于进行遗传监测并提供重要信息以帮助控制疾病。
