Singh R L, Khanna S K, Singh G B
Vet Hum Toxicol. 1987 Aug;29(4):300-4.
Inspite of its non-inclusion in the prescribed list of food colors, orange II is extensively employed to color a variety of foodstuffs. Oral LD50 value of orange II in both male and female rats was calculated to be more than 10.56 g/kg body weight. In short-term studies, animals were exposed to diets containing 0.0 (control), 0.1, 0.5 or 3.0% (w/w) of orange II, daily for 90 days. Hematological examination revealed a slight decrease in erythrocyte count and hemoglobin content, whereas leucocyte count, PCV, ESR, MCV, MCH and MCHC showed normal values. There was no change in the activities of LDH, GOT, GPT, alkaline/acid phosphatases and bioconstituents, lactic acid, cholesterol and protein in serum as well as in liver, indicating normal functioning of the liver. Histopathological examination of various body organs such as liver, heart, lung, kidney, testes, adrenal, stomach, large and small intestine presented normal appearance. Animals receiving 3.0% orange II showed marked splenomegaly and deposition of Perl's positive iron pigments. Testicular LDH, hyaluronidase and lactic acid did not reveal any deviation from controls, suggesting normal spermatogenic process. No changes in testicular cholesterol, fructose content of coagulating glands and dorso-lateral prostate, and activities of alkaline phosphatase in seminal vesicle and acid phosphatase in ventral prostate support normal androgenic status.
尽管橙黄 II 未被列入规定的食用色素清单中，但它仍被广泛用于给各种食品上色。经计算，橙黄 II 对雄性和雌性大鼠的经口半数致死量值均超过 10.56 克/千克体重。在短期研究中，动物每天被喂食含有 0.0（对照）、0.1、0.5 或 3.0%（w/w）橙黄 II 的饲料，持续 90 天。血液学检查显示红细胞计数和血红蛋白含量略有下降，而白细胞计数、红细胞压积、血沉、平均红细胞体积、平均红细胞血红蛋白含量和平均红细胞血红蛋白浓度均显示正常数值。血清以及肝脏中的乳酸脱氢酶、谷草转氨酶、谷丙转氨酶、碱性/酸性磷酸酶的活性和生物成分、乳酸、胆固醇和蛋白质均无变化，表明肝脏功能正常。对肝脏、心脏、肺、肾脏、睾丸、肾上腺、胃、大肠和小肠等各种身体器官进行组织病理学检查，结果显示外观正常。接受 3.0%橙黄 II 的动物出现明显的脾肿大以及 Perl 阳性铁色素沉积。睾丸中的乳酸脱氢酶、透明质酸酶和乳酸与对照组相比未显示任何偏差，表明生精过程正常。睾丸胆固醇、凝固腺和背外侧前列腺的果糖含量以及精囊中的碱性磷酸酶活性和腹侧前列腺中的酸性磷酸酶活性均无变化，支持正常的雄激素状态。
