Halliwell R E, Schemmer K R
Vet Immunol Immunopathol. 1987 Jun;15(3):203-13. doi: 10.1016/0165-2427(87)90083-3.
Biopsies taken of skin test sites from 10 dogs allergic to fleas were fixed in Karnovsky's fixative and embedded in methacrylate. One micron sections were stained with acid Giemsa for identification of basophils. This cell was identified in most biopsies taken at intervals from 1 hour to 48 hours post-injection. The proportion was highest between 4 hours and 18 hours and had substantially fallen by 48 hours. The highest number of basophils recorded as a percentage of the inflammatory infiltrate was 22.1%, with biopsies from 7 of the 10 dogs showing in excess of 10% basophils at some point. The results suggest that cutaneous basophil hypersensitivity may play an immunopathogenic role in flea-bite hypersensitivity in dogs.
从10只对跳蚤过敏的犬只的皮肤试验部位采集活检样本，用卡诺夫斯基固定液固定，然后包埋于甲基丙烯酸酯中。制作1微米厚的切片，用酸性吉姆萨染色以鉴定嗜碱性粒细胞。在注射后1小时至48小时的不同时间采集的大多数活检样本中都能识别出这种细胞。比例在4小时至18小时之间最高，到48小时时已大幅下降。记录到的嗜碱性粒细胞占炎症浸润细胞的最高百分比为22.1%，10只犬中有7只的活检样本在某些时间点显示嗜碱性粒细胞超过10%。结果表明，皮肤嗜碱性粒细胞超敏反应可能在犬只跳蚤叮咬超敏反应中起免疫致病作用。
