Garcia-Melo Luis Fernando, Morales-Rodríguez Miguel, Madrigal-Bujaidar Eduardo, Madrigal-Santillán Eduardo O, Morales-González José Antonio, Pineda Cruces Rosa Natali, Campoy Ramírez Jorge Alfredo, Damian-Matsumura Pablo, Tellez-Plancarte Alexandro, Batina Nikola, Álvarez-González Isela
Division de Ingeniería en Nanotecnología, Universidad Politécnica del Valle de México, Av. Mexiquense s/n esquina Av. Universidad Politécnica, Tultitlan Estado de México, CP 54910, Mexico.
Laboratorio de Nanotecnología e Ingeniería Molecular Área Electroquímica, Departamento de Química, CBI, Universidad Autónoma Metropolitana-Iztapalapa (UAM-I), Av. San Rafael Atlixco 186, Iztapalapa, CP 09340, México City, Mexico.
J Anal Methods Chem. 2022 Oct 17;2022:6575140. doi: 10.1155/2022/6575140. eCollection 2022.
In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the gene in the concentration range of 10 fM to 1 M with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the oncogene.
在科学文献中，已有记载表明电化学基因传感器是新型分析工具，在识别因遗传和表观遗传改变导致的致癌过程以及因病毒或细菌引起的传染病方面具有经证实的临床诊断潜力。在本研究中，我们描述了一种用于识别k12p.1突变的电化学基因传感器的构建；它基于使用丝网印刷金电极（SPGE）、循环伏安法（CV）和原子力显微镜（AFM），用于监测通过功能化纳米结构表面的电子转移以及相应的形态变化。该基因传感器的灵敏度在10 fM至1 M的浓度范围内对该基因的k12p.1突变识别呈线性响应，在存在作为DNA嵌入剂和杂交反应指示剂的阿霉素（Dox）时检测限为7.96 fM。因此，所开发的电化学基因传感器可用于识别与该癌基因相关的疾病。
