Chemical and Bioprocess Engineering Department, School of Engineering, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, P.O. Box 306, Santiago 7820436, Chile.
Department of Metabolism and Nutrition, Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), Calle José Antonio Novais, 10, Madrid 28040, Spain.
Food Chem. 2023 Feb 15;402:134472. doi: 10.1016/j.foodchem.2022.134472. Epub 2022 Sep 30.
An efficient macroporous resin purification process was designed to obtain food-grade phlorotannin-rich extracts from the seaweeds Durvillaea incurvata and Lessonia spicata. Phlorotannins were profiled to relate structures with the α-glucosidase inhibitory activity of the extracts. Liquid chromatography-mass spectrometry was applied for tentative identification. The best phlorotannin purification performance was achieved with HP-20 resin and elution with 80% v/v ethanol. This is the first study that demonstrates the effectivity of HP-20 resin for removing potentially toxic elements (As, Cd) from seaweed extracts. Fucols/phlorethols/fucophlorethols isomers up to 4 phloroglucinol units (PGU) were the most representative phlorotannins. High molecular weight species (11-21 PGU), although in low abundances, were detected in D. incurvata for the first time. Eckols, carmalols, fuhalols, phenolic acids, and flavonoids were also detected. Some extracts' potent α-glucosidase inhibitory activities were related to their high phlorotannin abundances, the presence of phlorotannins of a high degree of polymerization, and the phlorotannins class.
设计了一种高效的大孔树脂纯化工艺，从皱波角叉菜和长茎葡萄蕨藻中获得食品级富含岩藻多酚的提取物。对岩藻多酚进行了分析，以研究其结构与提取物中α-葡萄糖苷酶抑制活性的关系。采用液相色谱-质谱联用技术进行了初步鉴定。用 HP-20 树脂和 80%（v/v）乙醇洗脱，获得了最佳的岩藻多酚纯化效果。这是首次研究表明 HP-20 树脂可有效去除海藻提取物中的潜在有毒元素（As、Cd）。四聚体到七聚体的岩藻酚/菲三醇/岩藻菲三醇异构体是最具代表性的岩藻多酚。首次在皱波角叉菜中检测到高分子量物质（11-21 个 PGU），尽管含量较低。还检测到了 Eckols、Carmalols、Fuhalols、酚酸和类黄酮。一些提取物具有很强的α-葡萄糖苷酶抑制活性，这与其岩藻多酚含量高、高聚合度岩藻多酚的存在以及岩藻多酚的类别有关。
