Li Yu-Hang, Ren Qian-Qian, Wang Jin-Peng, Wang Xing-Ping, Luoreng Zhuo-Ma, Ma Yun, Wei Da-Wei
School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
School of Agriculture, Ningxia University, Yinchuan, China; Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
Res Vet Sci. 2022 Dec 31;153:57-60. doi: 10.1016/j.rvsc.2022.10.011. Epub 2022 Oct 18.
MicroRNAs (miRNAs) are involved in the regulation of a variety of biological processes. However, the research on the regulatory role of bovine mammary epithelial cells (bMECs) is scarce. To date, there are no reports about the role of miR-199a-3p in bMECs. In this study, RNA sequencing (RNA-seq) technology was used to detect the transcriptomes of the miR-199a-3p overexpression and negative control (NC) groups of bMECs. Then, the screening and functional annotation of differentially expressed genes (DEGs) were conducted. The results showed that there were 140 DEGs (109 up-regulated and 31 down-regulated) in the miR-199a-3p overexpression group. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that the DEGs might regulate the immune and inflammatory responses via the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway, transforming growth factor-beta (TGF-β) signaling pathway, and interleukin-17 (IL-17) signaling pathway, which revealed that miR-199a-3p might participate in regulating bMECs inflammation via affecting the expression of related genes and the above signaling pathways. This study may provide a new reference for potential therapeutic targets of cow mastitis.
微小RNA（miRNA）参与多种生物学过程的调控。然而，关于牛乳腺上皮细胞（bMECs）调控作用的研究却很少。迄今为止，尚无关于miR-199a-3p在bMECs中作用的报道。在本研究中，采用RNA测序（RNA-seq）技术检测bMECs的miR-199a-3p过表达组和阴性对照组（NC）的转录组。然后，对差异表达基因（DEGs）进行筛选和功能注释。结果显示，miR-199a-3p过表达组中有140个DEGs（109个上调，31个下调）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析结果表明，这些DEGs可能通过磷脂酰肌醇3-激酶（PI3K）/AKT信号通路、转化生长因子-β（TGF-β）信号通路和白细胞介素-17（IL-17）信号通路调节免疫和炎症反应，这表明miR-199a-3p可能通过影响相关基因的表达和上述信号通路参与调节bMECs炎症。本研究可能为奶牛乳腺炎潜在治疗靶点提供新的参考。
