Centro de Investigaciones Biológicas del Noroeste, S. C (CIBNOR), 23096, La Paz, Mexico.
Centro de Investigaciones Biológicas del Noroeste, S. C (CIBNOR), 23096, La Paz, Mexico; Hospital General de Zona No.1. Instituto Mexicano del Seguro Social, 23060, La Paz, Mexico.
Microb Pathog. 2022 Dec;173(Pt A):105800. doi: 10.1016/j.micpath.2022.105800. Epub 2022 Oct 26.
Few studies have analyzed the indicators of oxidative stress in marine mammals following exposure to lipopolysaccharides (LPS); sex and maturity-related differences have not been explored. The objective of this study was to compare the indicators of oxidative stress following exposure to LPS for 24 and 48 h in isolated Pacific bottlenose dolphin (Tursiops truncatus; N = 12) leukocytes in relation to sex and maturity stage, using spectrophotometry. Following 48 h under experimental conditions (10 μg LPS mL), the leukocytes from males (n = 5) produced significantly more superoxide radical (O; F (1, 8) = 13.965, p = 0.006) and displayed significantly greater activities of catalase (CAT; F (1, 8) = 9.465, p = 0.015) and glutathione S-transferase (GST; p = 0.028) compared to the leukocytes from females (n = 7). Following 48 h under experimental conditions, maturity-stage did not significantly influence the indicators of oxidative stress. Mature bottlenose dolphins (n = 7) had a significantly higher average daily dietary intake compared to immature bottlenose dolphins (n = 5; F (1, 10) = 5.825, p = 0.036). These results suggest that sex-related strategies for coping with a proinflammatory challenge may be present within the leukocytes from bottlenose dolphins, while potential maturity stage-related strategies require further investigation.
很少有研究分析过海洋哺乳动物在接触脂多糖 (LPS) 后氧化应激的指标；也没有探讨过性别和成熟度相关的差异。本研究的目的是使用分光光度法比较在 LPS 暴露 24 和 48 小时后，与性别和成熟阶段有关的分离太平洋瓶鼻海豚 (Tursiops truncatus；N=12) 白细胞中的氧化应激指标。在实验条件下 (10μg LPS mL) 经过 48 小时后，雄性 (n=5) 的白细胞产生的超氧自由基 (O；F(1, 8)=13.965，p=0.006) 明显更多，并且 CAT (F(1, 8)=9.465，p=0.015) 和谷胱甘肽 S-转移酶 (GST；p=0.028) 的活性明显更高。与雌性 (n=7) 的白细胞相比。在实验条件下经过 48 小时后，成熟度阶段对氧化应激指标没有显著影响。成熟的瓶鼻海豚 (n=7) 的平均每日膳食摄入量明显高于不成熟的瓶鼻海豚 (n=5；F(1, 10)=5.825，p=0.036)。这些结果表明，在瓶鼻海豚的白细胞中可能存在与性别相关的应对促炎挑战的策略，而潜在的成熟阶段相关策略需要进一步研究。
