蚕茧水醇提取物对链脲佐菌素诱导的糖尿病雄性大鼠下丘脑 - 垂体 - 性腺轴的影响

The Effects of Hydroalcoholic Extract of Silk Cocoon on Hypothalamic-Pituitary -Gonadal Axis in Streptozotocin-Induced Diabetic Male Rats.

作者信息

Ahi Salma, Ebrahimi Fatemeh, Abedi Hassan Ali, Kargar Jahromi Hossein, Zarei Safar

机构信息

Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.

Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.

出版信息

Autoimmune Dis. 2022 Oct 19;2022:7916159. doi: 10.1155/2022/7916159. eCollection 2022.

DOI:10.1155/2022/7916159

PMID:36311276

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9605846/

Abstract

BACKGROUND

Diabetes mellitus impairs the reproductive system by damaging the glands and changing their function and hormone secretions. Given the previous studies on medical properties of silk cocoon, the aim of this study was to investigate the effect of the hydroalcoholic extract of silk cocoon on pituitary-gonadal axis hormones and the testis changes in diabetic male rats.

METHODS

In this experimental study, 35 male rats were divided into 5 equal groups. Control (C), nontreated diabetic rats (DNT1), and experimental diabetic rats treated (DT1) with a silk cocoon extract at concentrations of 200, 400, and 800 mg/kg for 56 days. Diabetes was induced by an injection of streptozotocin. Blood sampling was performed by the tail and heart after fasting. Body weight, serum levels of glucose, prolactin, leptin, inhibin A, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH were measured along with the testis weight and diameter as the outcome of the study. Data were analyzed by SPSS version 20.

RESULTS

Investigation of hormonal factors indicated that all diabetic groups had higher prolactin, inhibin A levels than those in C group and lower leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels than controls. Silk cocoon treatment significantly decreased prolactin and inhibin in comparison of DNT1 group. While there was a significant increase in leptin, IGF-2, activin A, insulin, LH, testosterone, FSH, and GnRH levels compared with DNT1 ( < 0.05). A significant decrease in both the testis weights and diameters was observed in diabetic male rats compared to controls ( < 0.05). While silk cocoon treatment improved gonadal weight, the diameter of tunica albuginea, and seminiferous tubules as long as increased in numbers of spermatocytes and Sertoli-Leydig cells. Spermatogonia, spermatocyte, spermatid, spermatozoid, Sertoli cells, and Leydig cell count were significantly lower in the DNT1 group in comparison with the control group, while all groups receiving the highest dose of SC800 mg/kg daily had a higher count of cells than the DNT1 group.

CONCLUSION

It seems that silk cocoon treatment decreases the effects of diabetes on hypothalamic-pituitary-gonadal axis.

摘要

背景

糖尿病通过损害腺体、改变其功能和激素分泌来损害生殖系统。鉴于先前关于蚕茧医学特性的研究，本研究的目的是探讨蚕茧水醇提取物对糖尿病雄性大鼠垂体 - 性腺轴激素及睾丸变化的影响。

方法

在本实验研究中，35只雄性大鼠被分为5个相等的组。对照组（C）、未治疗的糖尿病大鼠（DNT1）以及分别用浓度为200、400和800 mg/kg的蚕茧提取物治疗的实验性糖尿病大鼠（DT1），持续56天。通过注射链脲佐菌素诱导糖尿病。禁食后通过尾部和心脏进行采血。测量体重、血清葡萄糖、催乳素、瘦素、抑制素A、胰岛素样生长因子 - 2（IGF - 2）、激活素A、胰岛素、黄体生成素（LH）、睾酮、卵泡刺激素（FSH）和促性腺激素释放激素（GnRH）水平以及睾丸重量和直径作为研究结果。数据用SPSS 20版进行分析。

结果

激素因素研究表明，所有糖尿病组的催乳素、抑制素A水平均高于C组，而瘦素、IGF - 2、激活素A、胰岛素、LH、睾酮、FSH和GnRH水平均低于对照组。与DNT1组相比，蚕茧治疗显著降低了催乳素和抑制素水平。与DNT1组相比，瘦素、IGF - 2、激活素A、胰岛素、LH、睾酮、FSH和GnRH水平显著升高（P < 0.05）。与对照组相比，糖尿病雄性大鼠的睾丸重量和直径均显著降低（P < 0.05）。而蚕茧治疗改善了性腺重量、白膜直径和生精小管，同时增加了精母细胞和支持 - 莱迪希细胞的数量。与对照组相比，DNT1组的精原细胞、精母细胞、精子细胞、精子、支持细胞和莱迪希细胞计数显著降低，而每天接受最高剂量800 mg/kg蚕茧提取物的所有组的细胞计数均高于DNT1组。