Bertuzzi Sara, Peccati Francesca, Serna Sonia, Artschwager Raik, Notova Simona, Thépaut Michel, Jiménez-Osés Gonzalo, Fieschi Franck, Reichardt Niels C, Jiménez-Barbero Jesús, Ardá Ana
Basque Research & Technology Alliance (BRTA), Chemical Glycobiology Group, CIC bioGUNE, Bizkaia Technology Park, Building 800, 48160 Derio, Bizkaia, Spain.
Basque Research & Technology Alliance (BRTA), Computational Chemistry Group, CIC bioGUNE, Bizkaia Technology Park, Building 800, 48160 Derio, Bizkaia, Spain.
ACS Cent Sci. 2022 Oct 26;8(10):1415-1423. doi: 10.1021/acscentsci.2c00719. Epub 2022 Sep 20.
The molecular recognition features of LSECtin toward asymmetric N-glycans have been scrutinized by NMR and compared to those occurring in glycan microarrays. A pair of positional glycan isomers (LDN3 and LDN6), a nonelongated GlcNAc4Man3 N-glycan (G0), and the minimum binding epitope (the GlcNAcβ1-2Man disaccharide) have been used to shed light on the preferred binding modes under both experimental conditions. Strikingly, both asymmetric LDN3 and LDN6 N-glycans are recognized by LSECtin with similar affinities in solution, in sharp contrast to the results obtained when those glycans are presented on microarrays, where only LDN6 was efficiently recognized by the lectin. Thus, different results can be obtained using different experimental approaches, pointing out the tremendous difficulty of translating results to the environment.
LSECtin对不对称N-聚糖的分子识别特征已通过核磁共振（NMR）进行了仔细研究，并与聚糖微阵列中的情况进行了比较。一对位置聚糖异构体（LDN3和LDN6）、一种未延长的GlcNAc4Man3 N-聚糖（G0）以及最小结合表位（GlcNAcβ1-2Man二糖）已被用于阐明两种实验条件下的优选结合模式。令人惊讶的是，在溶液中，不对称的LDN3和LDN6 N-聚糖都能被LSECtin以相似的亲和力识别，这与这些聚糖在微阵列上呈现时的结果形成鲜明对比，在微阵列上只有LDN6能被凝集素有效识别。因此，使用不同的实验方法可能会得到不同的结果，这表明将结果转化到生理环境中存在巨大困难。
