Cao Da, Barbier Francois, Yoneyama Kaori, Beveridge Christine A
ARC Centre of Excellence for Plant Success in Nature and Agriculture, School of Biological Sciences, The University of Queensland, St Lucia, 4072, Australia.
Graduate School of Agriculture, Ehime University, Matsuyama, Japan.
Bio Protoc. 2022 Oct 5;12(19). doi: 10.21769/BioProtoc.4524.
The quantification of plant hormones and related gene expression is essential to improve the understanding of the molecular regulation of plant growth and development. However, plant hormone quantification is still challenging due to extremely low endogenous levels and high chemical diversity. In this study, we present a convenient extraction protocol that enables the simultaneous extraction of both phytohormones and RNA from the same sample in a small quantity (approximately 10 mg). Using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS), this protocol provides a method to quantify 13 phytohormones and their derivatives from four classes (cytokinin, auxin, abscisic acid, and gibberellin) at the speed of 14 min per sample.
植物激素的定量分析以及相关基因表达对于增进对植物生长发育分子调控的理解至关重要。然而，由于植物激素内源性水平极低且化学多样性高，其定量分析仍然具有挑战性。在本研究中，我们提出了一种便捷的提取方案，该方案能够从少量（约10毫克）同一样本中同时提取植物激素和RNA。利用超高效液相色谱串联质谱法（UPLC-MS/MS），此方案提供了一种方法，可在每个样本14分钟的速度下对来自四类（细胞分裂素、生长素、脱落酸和赤霉素）的13种植物激素及其衍生物进行定量分析。
