Holland M J, Holland J P
Biochemistry. 1978 Nov 14;17(23):4900-7. doi: 10.1021/bi00616a007.
Yeast poly(adenylic acid)-containing messenger ribonucleic acid isolated from two strains of Saccharomyces cerevisiae was fractionated by preparative polyacrylamide gel electrophoresis in the presence of formamide. Three messenger ribonucleic acids, present at high intracellular concentration, were electrophoretically eluted from the polyacrylamide gels and translated in a wheat germ cell-free extract. The in vitro synthesized polypeptides were identified by tryptic peptide analysis. Messenger ribonucleic acids coding for enolase and glyceraldehyde-3-phosphate dehydrogenase were isolated from commercially grown baker's yeast (strain F1), and messenger ribonucleic acid coding for phosphoglycerate kinase was isolated from Saccharomyces cerevisiae (ATCC 24657). Significant differences in the spectrum of abundant messenger ribonucleic acids isolated from commercially grown baker's yeast (strain F1) and strain 24657 were observed. When both strains were grown under identical conditions, however, the spectrum of messenger ribonucleic acid isolated from the cells is indistinguishable.
从两株酿酒酵母中分离得到的含酵母聚腺苷酸信使核糖核酸，在甲酰胺存在下通过制备型聚丙烯酰胺凝胶电泳进行分级分离。从聚丙烯酰胺凝胶中电泳洗脱了三种细胞内浓度较高的信使核糖核酸，并在小麦胚芽无细胞提取物中进行翻译。通过胰蛋白酶肽分析鉴定体外合成的多肽。编码烯醇化酶和甘油醛-3-磷酸脱氢酶的信使核糖核酸从商业种植的面包酵母（菌株F1）中分离得到，编码磷酸甘油酸激酶的信使核糖核酸从酿酒酵母（ATCC 24657）中分离得到。观察到从商业种植的面包酵母（菌株F1）和24657菌株中分离得到的丰富信使核糖核酸谱存在显著差异。然而，当两株菌在相同条件下生长时，从细胞中分离得到的信使核糖核酸谱无法区分。
