凸包在单分子定位显微镜中作为诊断工具
Convex hull as diagnostic tool in single-molecule localization microscopy.
作者信息
Ebert Vincent, Eiring Patrick, Helmerich Dominic A, Seifert Rick, Sauer Markus, Doose Sören
机构信息
Department of Biotechnology and Biophysics, Julius-Maximilians University, 97074 Würzburg, Germany.
出版信息
Bioinformatics. 2022 Dec 13;38(24):5421-5429. doi: 10.1093/bioinformatics/btac700.
MOTIVATION
Single-molecule localization microscopy resolves individual fluorophores or fluorescence-labeled biomolecules. Data are provided as a set of localizations that distribute normally around the true fluorophore position with a variance determined by the localization precision. Characterizing the spatial fluorophore distribution to differentiate between resolution-limited localization clusters, which resemble individual biomolecules, and extended structures, which represent aggregated molecular complexes, is a common challenge.
RESULTS
We demonstrate the use of the convex hull and related hull properties of localization clusters for diagnostic purposes, as a parameter for cluster selection or as a tool to determine localization precision.
AVAILABILITY AND IMPLEMENTATION
https://github.com/super-resolution/Ebert-et-al-2022-supplement.
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
动机
单分子定位显微镜可分辨单个荧光团或荧光标记的生物分子。数据以一组定位的形式提供，这些定位围绕真实荧光团位置呈正态分布，其方差由定位精度决定。区分分辨率受限的定位簇（类似于单个生物分子）和扩展结构（代表聚集的分子复合物）的空间荧光团分布特征是一项常见挑战。
结果
我们展示了利用定位簇的凸包及相关包壳属性进行诊断，作为簇选择的参数或确定定位精度的工具。
可用性和实现方式
https://github.com/super-resolution/Ebert-et-al-2022-supplement。
补充信息
补充数据可在《生物信息学》在线获取。
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