虾青素缓解棕榈酸诱导的猪卵母细胞成熟障碍。

Astaxanthin alleviates palmitic acid-induced hindrance of porcine oocyte maturation.

机构信息

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Reproductive Research Central Lab, Swine Research Institutes of TechBank Foods, Huaian, China.

出版信息

Reprod Domest Anim. 2022 Nov;57(11):1440-1449. doi: 10.1111/rda.14221. Epub 2022 Aug 7.

DOI:10.1111/rda.14221

PMID:36317481

Abstract

Increased palmitic acid (PA) levels have been found in females with reduced fertility due to metabolic disorders. However, effective antioxidant astaxanthin (AXE) might positively affect animal reproduction. Therefore, the present study was designed to evaluate the impact of a high concentration of PA on oocyte maturation and the possible protective effect of AXE against high PA concentration in pigs. Firstly, different concentrations (0.2, 0.5, 0.8 mM) of PA were conducted on in vitro maturation (IVM) of pig oocytes (PA0.2, PA0.5, and PA0.8), while no addition of PA was performed as the control group (Ctrl). Results showed that the cumulus cell expansion index (CCEI) was lower in PA0.5 and PA0.8 groups compared to Ctrl group (p < .05). In PA0.5 group, not only did the percentage of matured oocytes with the first polar body (PB1) reduced, that with more oocytes arrested at germinal vesicle (GV) stage (53.44% ± 7.16% vs. 20.93% ± 5.16%, p < .05), but also a higher number of transzonal projections (TZPs) was observed in PA0.5 than Ctrl group. Besides, supplement of PA resulted in a dose-dependent decrease in mitochondrial activity. Although no difference of lipid content was observed between PA0.5 and Ctrl groups, the lipid content was at a higher level in PA0.2 group than in the other three groups. Hence, concentration of 0.5 mM of PA was performed in the following experiments, and 2.5 μM AXE carried out to investigate the possible relief effects of PA (PA0.5 + AXE). Results showed that the percentage of matured oocytes with PB1 was higher in PA0.5 + AXE than in PA0.5 group (63.43% ± 1.50% vs. 55.33% ± 0.81%, p < .01), and ROS levels both in oocytes and their cumulus cells (CCs) reduced in PA0.5 + AXE when compared to PA0.5 group. In addition, the rate of CCs with apoptosis decreased in PA0.5 + AXE, and the expression level of caspase 3 and BAX was lower than PA0.5 group. In conclusion, the maturation of pig oocytes was inhibited by the high concentration of PA; however, this negative effect of PA-induced might be relieved by the supplement of AXE.

摘要

已有研究发现，由于代谢紊乱而导致生育能力下降的女性，其棕榈酸（PA）水平升高。然而，高效抗氧化虾青素（AXE）可能对动物繁殖有积极影响。因此，本研究旨在评估高浓度 PA 对卵母细胞成熟的影响，以及 AXE 对猪高 PA 浓度的可能保护作用。首先，我们在猪卵母细胞的体外成熟（IVM）中进行了不同浓度（0.2、0.5、0.8 mM）的 PA 处理（PA0.2、PA0.5 和 PA0.8），同时未添加 PA 的作为对照组（Ctrl）。结果表明，与对照组相比，PA0.5 和 PA0.8 组的卵丘细胞扩展指数（CCEI）较低（p <.05）。在 PA0.5 组中，不仅第一次极体（PB1）成熟的卵母细胞比例降低（53.44%±7.16%比 20.93%±5.16%，p <.05），而且处于生发泡（GV）阶段的卵母细胞数量更多（53.44%±7.16%比 20.93%±5.16%，p <.05），并且在 PA0.5 组中观察到的透明带突起（TZPs）数量高于对照组。此外，添加 PA 导致线粒体活性呈剂量依赖性下降。虽然 PA0.5 组和对照组之间的脂质含量没有差异，但 PA0.2 组的脂质含量高于其他三组。因此，在后续实验中采用了 0.5 mM 的 PA 浓度，并进行了 2.5 μM AXE 实验，以研究 PA 的可能缓解作用（PA0.5+AXE）。结果表明，与 PA0.5 组相比，PA0.5+AXE 组中具有 PB1 的成熟卵母细胞比例更高（63.43%±1.50%比 55.33%±0.81%，p <.01），并且卵母细胞及其卵丘细胞（CCs）中的 ROS 水平均降低。此外，PA0.5+AXE 组中 CCs 凋亡率降低，caspase 3 和 BAX 的表达水平低于 PA0.5 组。总之，高浓度 PA 抑制了猪卵母细胞的成熟，但 AXE 的补充可能缓解 PA 诱导的这种负面影响。