Park Suhyun, Kim Hyueyun, Woo Minna, Kim Minsuk
Department of Pharmacology, College of Medicine, Ewha Womans University, Seoul, 07804, Republic of Korea.
Department of Medicine, Toronto General Hospital Research Institute and Division of Endocrinology and Metabolism, University Health Network, University of Toronto, Toronto, ON, Canada.
J Biol Eng. 2022 Nov 1;16(1):29. doi: 10.1186/s13036-022-00308-6.
Chronic myeloid leukemia is generally required bone marrow biopsy for diagnosis. Although examining peripheral blood is less invasive, it has not been fully validated as a routine diagnostic test due to suboptimal sensitivity. To overcome this limitation, a number of methodologies based on microfluidics have been developed for sorting circulating tumor cells from peripheral blood of patients with leukemia.In order to develop a more convenient method, we designed an analysis protocol using motion microscopy that amplifies cellular micro motions in a captured video by re-rendering pixels to generate extreme magnified visuals. Intriguingly, no fluctuations around leukemic myeloblasts were observed with a motion microscope at any wavelength of 0-10 Hz. However, use of 0.05% hyaluronic acid, one type of non-newtonian fluid, demonstrated fluctuations around leukemic myeloblasts under conditions of 25 μm/s and 0.5-1.5 Hz with a motion microscope.Thus, the non-invasive detection of leukemic myeloblasts can offer a valuable supplementary diagnostic tool for assessment of drug efficacy for monitoring patients with chronic myeloid leukemia.
慢性髓性白血病通常需要进行骨髓活检来诊断。虽然检测外周血的侵入性较小，但由于敏感性欠佳，它尚未作为常规诊断测试得到充分验证。为克服这一局限性，已开发出多种基于微流控技术的方法，用于从白血病患者外周血中筛选循环肿瘤细胞。为了开发一种更便捷的方法，我们设计了一种使用运动显微镜的分析方案，该方案通过重新渲染像素来放大捕获视频中的细胞微运动，以生成极度放大的图像。有趣的是，在0至10赫兹的任何波长下，使用运动显微镜均未观察到白血病成髓细胞周围有波动。然而，使用一种非牛顿流体0.05%透明质酸，在运动显微镜下，在25微米/秒和0.5至1.5赫兹的条件下，白血病成髓细胞周围出现了波动。因此，白血病成髓细胞的非侵入性检测可为评估慢性髓性白血病患者的药物疗效提供一种有价值的辅助诊断工具。
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