阴道毛滴虫的分泌排泄产物导致体外培养的小鼠精子凋亡。

Excretory-Secretory Products of Trichomonas vaginalis Cause Apoptosis in Mouse Sperm in Vitro.

机构信息

Deptartment of Obstetrics & Gynecology, College of Medicine, Hanyang University, Seoul 04763, Korea.

Laboratory of Reproductive Endocrinology, Deptartment. of Anatomy & Cell Biology, College of Medicine, Hanyang University, Seoul 04763, Korea.

出版信息

Korean J Parasitol. 2022 Oct;60(5):357-360. doi: 10.3347/kjp.2022.60.5.357. Epub 2022 Oct 21.

DOI:10.3347/kjp.2022.60.5.357

PMID:36320113

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9633159/

Abstract

Excretory-secretory products (ESP) of T. vaginalis have been shown to inhibit sperm motility, viability, and functional integrity, leading to a decreased fertilization rate in vitro. This study investigated whether T. vaginalis induce apoptosis and ultrastructural changes of sperm using flow cytometry and electron microscopy. Incubation of sperm with T. vaginalis ESP increased phosphatidylserine externalization and DNA fragmentation, and decreased mitochondrial membrane potential. Transmission electron microscopy of sperm incubated with ESP revealed abnormal features such as distorted heads, broken necks, and acrosomes exocytosis. This is the first report that demonstrates a direct impact of T. vaginalis ESP on sperm apoptosis and architecture in vitro.

摘要

阴道毛滴虫的排泄分泌产物（ESP）已被证明可抑制精子的运动性、活力和功能完整性，导致体外受精率降低。本研究通过流式细胞术和电子显微镜观察阴道毛滴虫是否诱导精子凋亡和超微结构变化。与阴道毛滴虫 ESP 孵育的精子，其磷脂酰丝氨酸外翻和 DNA 片段化增加，线粒体膜电位降低。用 ESP 孵育的精子的透射电子显微镜显示出异常特征，如头部扭曲、颈部断裂和顶体溢出。这是首次报道阴道毛滴虫 ESP 对体外精子凋亡和结构有直接影响。