Lin Huibin, Yang Chaoyong, Wang Wei
Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200127 China
The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Key Laboratory for Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University Xiamen 361005 China.
RSC Chem Biol. 2022 Aug 5;3(10):1198-1208. doi: 10.1039/d2cb00086e. eCollection 2022 Oct 5.
Because of its high involvement in antibiotic therapy and the emergence of drug-resistance, the chemical structure and biosynthesis of bacterial peptidoglycan (PGN) have been some of the key topics in bacteriology for several decades. Recent advances in the development of fluorescent or bio-orthogonal stem peptide-mimicking probes for PGN-labeling have rekindled the interest of chemical biologists and microbiologists in this area. The structural designs, bio-orthogonal features and flexible uses of these peptide-based probes allow directly assessing, not only the presence of PGN in different biological systems, but also specific steps in PGN biosynthesis. In this review, we summarize the design rationales, functioning mechanisms, and microbial processes/questions involved in these PGN-targeting probes. Our perspectives on the limitations and future development of these tools are also presented.
由于其在抗生素治疗中的高度参与以及耐药性的出现，细菌肽聚糖（PGN）的化学结构和生物合成在几十年来一直是细菌学的一些关键主题。用于PGN标记的荧光或生物正交茎肽模拟探针开发的最新进展重新激发了化学生物学家和微生物学家对该领域的兴趣。这些基于肽的探针的结构设计、生物正交特性和灵活用途不仅允许直接评估不同生物系统中PGN的存在，还能评估PGN生物合成中的特定步骤。在本综述中，我们总结了这些靶向PGN的探针的设计原理、作用机制以及涉及的微生物过程/问题。我们还提出了对这些工具的局限性和未来发展的看法。
