Department of Public Health, 74496Jining Medical University, Jining, China.
Toxicol Ind Health. 2022 Dec;38(12):810-818. doi: 10.1177/07482337221136949. Epub 2022 Nov 2.
Alveolar macrophages play a vital role in the development of acute silicosis, but the dynamic changes of M1 and/or M2 phenotypes have not been elucidated. In this study, acute silicosis models of rat were established by a one-time dusting method, and the rats were sacrificed after 1, 3, 7, 14, and 28 days. The polarity states of macrophages were assessed by measuring the M1/M2 marker genes of alveolar macrophages and the M1/M2 marker proteins in bronchoalveolar lavage fluid. The pathological changes of lung tissues were examined with hematoxylin and Eosin and Masson's trichrome staining. Our results showed that in the early stages, alveolar macrophages were mainly polarized into M1; with time, the polarization of M2 gradually became dominant. Microscopic sections showed significant pathological responses of inflammation and fibrosis. This work suggested that the alteration of alveolar macrophage polarization was involved in the lung pathologic responses to acute silicosis.
肺泡巨噬细胞在急性矽肺的发展中起着至关重要的作用，但 M1 和/或 M2 表型的动态变化尚未阐明。在这项研究中，通过一次性粉尘法建立了大鼠急性矽肺模型，大鼠分别在 1、3、7、14 和 28 天后处死。通过测量肺泡巨噬细胞的 M1/M2 标记基因和支气管肺泡灌洗液中的 M1/M2 标记蛋白来评估巨噬细胞的极性状态。用苏木精和伊红以及 Masson 三色染色法检查肺组织的病理变化。我们的结果表明，在早期阶段，肺泡巨噬细胞主要向 M1 极化；随着时间的推移，M2 的极化逐渐占主导地位。显微镜切片显示出明显的炎症和纤维化的病理反应。这项工作表明，肺泡巨噬细胞极化的改变参与了急性矽肺对肺的病理反应。
