Butler A P, McDonald F F
Biochem Biophys Res Commun. 1987 Sep 15;147(2):809-17. doi: 10.1016/0006-291x(87)91002-3.
The contribution of changes in mRNA levels to the induction of ornithine decarboxylase (ODC) by 12-O-tetradecanoylphorbol-13-acetate (TPA) in rat H35 hepatoma cells was analyzed by Northern blot and quantitative dot blot hybridization. ODC mRNA accumulated rapidly in TPA-treated cultures. The increase in message was transient, reaching a peak within about 3 h, then declining to control levels after 18 h. Maximal accumulation of ODC-specific mRNA varied from 3- to 8-fold above control. The TPA dose-response for ODC message accumulation was half-maximal at approximately 0.18 microM TPA. The increase was completely blocked by actinomycin D, suggesting that TPA stimulates the transcription of ODC genes. Inhibition of protein synthesis by cycloheximide (10 micrograms/ml) led to a superinduction of ODC mRNA in the presence of TPA, which suggested that a short-lived protein may be responsible for negative control of ODC expression.
通过Northern印迹法和定量斑点印迹杂交法分析了12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）诱导大鼠H35肝癌细胞中鸟氨酸脱羧酶（ODC）时mRNA水平变化的作用。在TPA处理的培养物中，ODC mRNA迅速积累。信息的增加是短暂的，在约3小时内达到峰值，然后在18小时后降至对照水平。ODC特异性mRNA的最大积累量比对照高3至8倍。ODC信息积累的TPA剂量反应在约0.18 microM TPA时达到半数最大效应。放线菌素D完全阻断了这种增加，表明TPA刺激了ODC基因的转录。在存在TPA的情况下，用环己酰亚胺（10微克/毫升）抑制蛋白质合成导致ODC mRNA的超诱导，这表明一种寿命短暂的蛋白质可能负责ODC表达的负调控。
