College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.
Huaikou High School, Jintang County, Chengdu, Sichuan 610000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Feb 15;287(Pt 1):122056. doi: 10.1016/j.saa.2022.122056. Epub 2022 Oct 28.
We have found that tris (2,2'-bipyridyl) ruthenium (II) (Ru(bpy)) possesses a high photo-induced oxidase-like activity and is capable of catalyzing the color reaction of 3,3',5,5'-tetramethylbenzidine (TMB) with dissolved oxygen. Ru(bpy) has a catalytic constant (K) that is twice as high as that of fluorescein, 170 and 275-fold higher than that of 9-mesityl-10-methyl acridine and Eosin Y, respectively. Electron spin resonance spectroscopy (ESR) and radical scavenging experiments have verified the major active radicals involved in the color reaction are •OH. A colorimetric biothiol assay has been successfully developed for the oxidase-like activity of Ru(bpy) can be suppressed by sulfhydryl compounds. A linear dependence between the decrease in absorbance and the logarithm of thiol concentrations can be found ranging from 5.0 to 50 μM, with a detection limit of 1.0 μM. This work reveals a new oxidase mimic with high catalytic activity and will facilitate the utilization of this oxidase mimic in biochemical analysis.
我们发现三（2,2'-联吡啶）钌（II）（Ru(bpy)）具有高的光诱导氧化酶样活性，并能够催化 3,3',5,5'-四甲基联苯胺（TMB）与溶解氧的显色反应。Ru(bpy) 的催化常数（K）是荧光素的两倍，分别比 9-均三甲苯基-10-甲基吖啶和曙红 Y 高 170 倍和 275 倍。电子自旋共振光谱（ESR）和自由基清除实验验证了参与显色反应的主要活性自由基是 •OH。已经成功开发了一种比色生物硫醇测定法，因为巯基化合物可以抑制 Ru(bpy) 的氧化酶样活性。在 5.0 至 50 μM 的范围内，吸光度的降低与硫醇浓度的对数之间存在线性关系，检测限为 1.0 μM。这项工作揭示了一种具有高催化活性的新型氧化酶模拟物，将有助于在生化分析中利用这种氧化酶模拟物。
