Alsowayeh Noorah, Albutti Aqel
Department of Biology, College of Education (Majmaah), Majmaah University, Al Majmaah, Saudi Arabia.
Department of Medical Biotechnology, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
Front Med (Lausanne). 2022 Oct 18;9:945938. doi: 10.3389/fmed.2022.945938. eCollection 2022.
, a gram-negative soil-dwelling bacterium, is primarily considered a causative agent of melioidosis infection in both animals and humans. Despite the severity of the disease, there is currently no licensed vaccine on the market. The development of an effective vaccine against could help prevent the spread of infection. The purpose of this study was to develop a multi-epitope-based vaccine against using advanced bacterial pan-genome analysis. A total of four proteins were prioritized for epitope prediction by using multiple subtractive proteomics filters. Following that, a multi-epitopes based chimeric vaccine construct was modeled and joined with an adjuvant to improve the potency of the designed vaccine construct. The structure of the construct was predicted and analyzed for flexibility. A population coverage analysis was performed to evaluate the broad-spectrum applicability of . The computed combined world population coverage was 99.74%. Molecular docking analysis was applied further to evaluate the binding efficacy of the designed vaccine construct with the human toll-like receptors-5 (TLR-5). Furthermore, the dynamic behavior and stability of the docked complexes were investigated using molecular dynamics simulation, and the binding free energy determined for Vaccine-TLR-5 was delta total -168.3588. The docking result revealed that the vaccine construct may elicit a suitable immunological response within the host body. Hence, we believe that the designed vaccine could be helpful for experimentalists in the formulation of a highly effective vaccine for .
伯克霍尔德菌是一种革兰氏阴性土壤细菌，主要被认为是动物和人类类鼻疽感染的病原体。尽管该疾病很严重，但目前市场上没有获批的疫苗。开发一种针对伯克霍尔德菌的有效疫苗有助于预防感染传播。本研究的目的是利用先进的细菌泛基因组分析开发一种基于多表位的伯克霍尔德菌疫苗。通过使用多种消减蛋白质组学过滤器，共筛选出四种蛋白质用于表位预测。随后，构建了一种基于多表位的嵌合疫苗构建体，并与佐剂结合以提高设计的疫苗构建体的效力。对构建体的结构进行了预测并分析了其灵活性。进行了群体覆盖率分析以评估伯克霍尔德菌的广谱适用性。计算得出的全球人口综合覆盖率为99.74%。进一步应用分子对接分析来评估设计的疫苗构建体与人类 Toll 样受体 5（TLR - 5）的结合效力。此外，使用分子动力学模拟研究了对接复合物的动态行为和稳定性，确定疫苗 - TLR - 5 的结合自由能为总 delta -168.3588。对接结果表明，该疫苗构建体可能在宿主体内引发合适的免疫反应。因此，我们认为设计的伯克霍尔德菌疫苗可能有助于实验人员制备一种针对伯克霍尔德菌的高效疫苗。
