Wang Bin, Fan Xiaoxuan, Sun Qianwen, Zhang Qingxiang, Kong Yue, Meng Qingyan, Zhao Dongsheng, Mao Beibei, Liu Yan, Zhao Pan, Zhang Lu, Yan Peizheng
College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, Shandong 250355, China.
Evid Based Complement Alternat Med. 2022 Oct 25;2022:3943343. doi: 10.1155/2022/3943343. eCollection 2022.
Asthma is a common chronic respiratory disease characterized by wheezing and shortness of breath. Its risk factors include genetic and acquired factors. The acquired factors are closely related to the environment, especially cold conditions. Autophagy plays a regulatory role in asthma. Therefore, we hypothesized that asthma can be controlled by drug intervention at the autophagy level under cold conditions. The Xiaoqinglong decoction (XQLT) was freeze-dried. The compounds in the freeze-dried powder were identified and quantified using reference standards via the high-performance liquid chromatography method. Ovalbumin (OVA)-sensitized rats were subjected to cold stimulation. The effect of cold stimulation on autophagy levels was determined, and it was confirmed that cold stimulation affected autophagy. The effects and mechanisms of XQLT in an asthmatic rat model (OVA-sensitized rats stimulated with cold) were explored. The concentrations of paeoniflorin, liquiritin, trans-cinnamic acid, glycyrrhizic acid, 6-gingerol, schisandrol A, and asarinin in XQLT freeze-dried powder were 14.45, 3.85, 1.03, 3.93, 0.59, 0.24, and 0.091 mg/g, respectively. Cold stimulation is an important cause of asthma. The inflammatory factors in bronchoalveolar lavage fluid and serum were increased in the model group, accompanied by a decline in autophagy level. The treatment with XQLT increased the expression of autophagy genes and decreased the expression of inflammatory factors. Histological studies showed that XQLT improved inflammatory infiltration and collagen fiber deposition in the lungs of rats. XQLT intervention increased autophagy in asthmatic rats. Autophagy plays a role in phagocytosis and reduces the accumulation of abnormal metabolites in the body to reduce airway inflammation and promote asthma recovery.
哮喘是一种常见的慢性呼吸道疾病，其特征为喘息和呼吸急促。其危险因素包括遗传因素和后天因素。后天因素与环境密切相关，尤其是寒冷条件。自噬在哮喘中发挥调节作用。因此，我们假设在寒冷条件下，可通过自噬水平的药物干预来控制哮喘。将小青龙汤（XQLT）冻干。采用高效液相色谱法，使用对照品对冻干粉中的化合物进行鉴定和定量。对卵清蛋白（OVA）致敏的大鼠进行冷刺激。测定冷刺激对自噬水平的影响，并证实冷刺激影响自噬。探讨了XQLT在哮喘大鼠模型（OVA致敏大鼠经冷刺激）中的作用及机制。XQLT冻干粉中芍药苷、甘草苷、反式肉桂酸、甘草酸、6-姜酚、五味子醇甲和细辛脂素的浓度分别为14.45、3.85、1.03、3.93、0.59、0.24和0.091mg/g。冷刺激是哮喘的重要病因。模型组支气管肺泡灌洗液和血清中的炎症因子增加，同时自噬水平下降。XQLT治疗增加了自噬基因的表达，降低了炎症因子的表达。组织学研究表明，XQLT改善了大鼠肺部的炎症浸润和胶原纤维沉积。XQLT干预增加了哮喘大鼠的自噬。自噬在吞噬作用中发挥作用，减少体内异常代谢产物的积累，以减轻气道炎症并促进哮喘恢复。
