Department of Nephrology, Sinopharm Dongfeng General Hospital, Hubei University of Medicine, 442008 Shiyan, Hubei, China.
Arch Esp Urol. 2022 Oct;75(8):714-719. doi: 10.56434/j.arch.esp.urol.20227508.103.
Vascular calcification (VC) is a serious pathological manifestation of vascular terminals and is an important pathological basis of cardiovascular diseases in patients with end-stage renal disease (ESRD). This study explored the clinical value of serum microRNA-211-5p (miR-211-5p) in VC in patients with ESRD, this study was designed.
The relative expression of miR-211-5p in the serum of the calcified group and the non-calcified group was quantified by quantitative reverse transcription polymerase chain reaction. The clinical biochemical indexes and the expression differences in miR-211-5p expression in the serum of groups were compared by two independent samples -test or nonparametric test or one-way analysis of variance test. The receiver operating characteristic (ROC), Kaplan-Meier, and Cox regression analysis were carried out to analyze the predictive value of serum miR-211-5p in patients with ESRD.
The relative quantification of serum miR-211-5p was lessened in calcified group and gradually decreased with the progression of VC. Serum miR-211-5p had a high diagnostic accuracy in the diagnosis of VC progression in ESRD patients. Kaplan-Meier and Cox regression methods revealed that miR-211-5p might be an independent biomarker for prognosis of ESRD patients.
MiR-211-5p is a potential diagnostic and prognostic marker of VC in patients with ESRD.
血管钙化（VC）是血管末端的严重病理表现，是终末期肾病（ESRD）患者心血管疾病的重要病理基础。本研究旨在探讨血清微小 RNA-211-5p（miR-211-5p）在 ESRD 患者 VC 中的临床价值。
采用定量逆转录聚合酶链反应（qRT-PCR）定量检测钙化组和非钙化组血清中 miR-211-5p 的相对表达量。采用两独立样本 t 检验或非参数检验或单因素方差分析比较各组间血清 miR-211-5p 表达的临床生化指标和表达差异。采用受试者工作特征（ROC）曲线、Kaplan-Meier 法和 Cox 回归分析评估血清 miR-211-5p 对 ESRD 患者的预测价值。
钙化组血清 miR-211-5p 的相对定量降低，且随 VC 进展逐渐降低。血清 miR-211-5p 对 ESRD 患者 VC 进展的诊断具有较高的准确性。Kaplan-Meier 和 Cox 回归方法表明，miR-211-5p 可能是 ESRD 患者预后的独立生物标志物。
miR-211-5p 是 ESRD 患者 VC 的潜在诊断和预后标志物。
