Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi 221 005, India.
J Genet. 2022;101.
The pioneering studies carried out on heat shock-induced synthesis of specific proteins in the early 1970s did not identify any Hsp60 family protein in . By the early 1980s, although the members of Hsp60 family of heat shock proteins (Hsp) were identified in a wide range of eukaryotes as homologs of the bacterial GroEL, none was known in . The existence of the Hsp60 family protein was serendipitously revealed in in my laboratory in 1989. Contrary to the earlier reports that all tissues in flies display the canonical heat shock response, the larval Malpighian tubules (MT) did not show induction of any of the major Hsps but synthesis of a putative Hsp60 family protein was found to be the most abundant in this tissue. A few years later, we identified this MTspecific heat shock-induced protein to indeed be a member of the Hsp60/chaperonin family. The genome sequence projects subsequently revealed four putative gene sequences in the . genome. The present historical perspective chronicles contributions from my and other laboratories that unraveled several aspects of intriguing biology of the multiple genes in . , and highlights challenging questions awaiting future studies.
20 世纪 70 年代早期开展的有关热休克诱导特定蛋白质合成的开创性研究并未在 中鉴定出任何 Hsp60 家族蛋白。到 20 世纪 80 年代初，尽管在广泛的真核生物中鉴定出热休克蛋白（Hsp）的 Hsp60 家族成员是细菌 GroEL 的同源物，但在 中尚未发现任何成员。1989 年，我在实验室中偶然发现了 Hsp60 家族蛋白的存在。与先前所有组织在果蝇中都表现出典型热休克反应的报道相反，幼虫的马氏管（MT）并未诱导任何主要的 Hsps，但发现一种假定的 Hsp60 家族蛋白的合成在该组织中最为丰富。几年后，我们确定这种 MT 特异性热休克诱导蛋白确实是 Hsp60/伴侣素家族的一员。随后的基因组序列项目揭示了 中四个假定的 基因序列。本历史观点记载了我和其他实验室的贡献，这些贡献揭示了 中多个 基因的有趣生物学的几个方面，并强调了未来研究中有待解决的具有挑战性的问题。
