Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea.
Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul 08826, Republic of Korea.
Prog Neurobiol. 2022 Dec;219:102369. doi: 10.1016/j.pneurobio.2022.102369. Epub 2022 Oct 28.
Sensing smells of foods, prey, or predators determines animal survival. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca play a significant role in transducing odorant inputs to electrical activity. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Ano9-deficient mice had reduced olfactory behavioral sensitivity, electro-olfactogram signals, and neural activity in the olfactory bulb. In line with the difference in olfaction between birds and other vertebrates, chick ANO9 failed to respond to odorants, whereas chick CNGA2, a major transduction channel, showed greater responses to cAMP. Thus, we concluded that the signal amplification by ANO9 is important for mammalian olfactory transduction.
感知食物、猎物或捕食者的气味决定了动物的生存。嗅觉上皮（OE）中的嗅觉感觉神经元检测气味，其中 cAMP 和 Ca 在将气味输入转化为电活动方面发挥着重要作用。在这里，我们展示了一种被 cAMP/PKA 途径激活的阳离子通道 Anoctamin 9（ANO9）在 OE 中表达，并放大嗅觉信号。ANO9 缺陷小鼠的嗅觉行为敏感性、电嗅图信号和嗅球中的神经活动降低。与鸟类和其他脊椎动物之间嗅觉的差异一致，鸡 ANO9 对气味没有反应，而作为主要转导通道的鸡 CNGA2 对 cAMP 的反应更大。因此，我们得出结论，ANO9 的信号放大对于哺乳动物嗅觉转导很重要。
