Department of Cell and Molecular Biology, University of Southern Mississippi, Hattiesburg, MS, USA.
Space Biosciences Division, NASA Ames Research Center, Moffett Field, CA, USA.
Methods Mol Biol. 2023;2585:33-40. doi: 10.1007/978-1-0716-2760-0_5.
West Nile virus (WNV) is a single-stranded-RNA flavivirus that can cause neurological illnesses. The ability of WNV to cause neurological illnesses is dependent on the virus' ability to gain access into the brain. However, the mechanisms by which WNV enters the brain are elusive, with evidence that neutrophils act as vehicles for viral delivery. To determine the role of neutrophils in WNV central nervous system delivery, modified protocols for the isolation and migration of neutrophils from mouse bone marrow for in vitro WNV infection modeling is described.
西尼罗河病毒（WNV）是一种单链 RNA 黄病毒，可导致神经疾病。WNV 引起神经疾病的能力取决于该病毒进入大脑的能力。然而，WNV 进入大脑的机制尚不清楚，有证据表明中性粒细胞充当病毒输送的载体。为了确定中性粒细胞在 WNV 中枢神经系统输送中的作用，描述了用于从鼠骨髓中分离和迁移中性粒细胞进行体外 WNV 感染建模的改良方案。
