Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China.
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
Theriogenology. 2023 Jan 1;195:77-84. doi: 10.1016/j.theriogenology.2022.10.006. Epub 2022 Oct 7.
Embryonic mortality is considered to be one of the main reasons for reduced conception rates in the cattle industry. Insufficient endometrial receptivity is a major factor resulting in embryo implantation failure and losses. Apoptosis of endometrial epithelial cells is an important process during establishment of uterine receptivity and embryo implantation. The aim of this study was to explore the role of bta-miR-200b on endometrial epithelial cell apoptosis in cattle. Overexpression of bta-miR-200b upregulated the expression of proapoptotic gene BCL2 associated X, apoptosis regulator (BAX) and endometrial receptivity marker gene osteopontin (OPN) at mRNA and protein level in bovine endometrial epithelial cells. Moreover, overexpression of bta-miR-200b was able to inhibit proliferation and promote apoptosis of bovine endometrial epithelial cells by arresting the cell cycle at the G0/G1 phase. MYB Proto-Oncogene (MYB) was verified to be a target of bta-miR-200b in bovine endometrial epithelial cells using dual-luciferase reporter assay. Transfection of bta-miR-200b mimics decreased the mRNA and protein expression of MYB. Overexpression of MYB decreased the effect of bta-miR-200b on apoptosis of bovine endometrial epithelial cells. Our findings suggest that bta-miR-200b can affect the apoptosis of endometrial epithelial cells in cattle by targeting the MYB gene.
胚胎死亡率被认为是牛养殖业中受孕率降低的主要原因之一。子宫内膜容受性不足是导致胚胎着床失败和损失的主要因素。子宫内膜上皮细胞的凋亡是建立子宫容受性和胚胎着床的重要过程。本研究旨在探讨 bta-miR-200b 在牛子宫内膜上皮细胞凋亡中的作用。bta-miR-200b 的过表达上调了牛子宫内膜上皮细胞中促凋亡基因 BCL2 相关 X、凋亡调节因子（BAX）和子宫内膜容受性标记基因骨桥蛋白（OPN）的 mRNA 和蛋白水平。此外，bta-miR-200b 的过表达通过将细胞周期阻滞在 G0/G1 期，能够抑制牛子宫内膜上皮细胞的增殖并促进其凋亡。双荧光素酶报告基因检测验证了 MYB 原癌基因（MYB）是牛子宫内膜上皮细胞中 bta-miR-200b 的靶基因。bta-miR-200b 模拟物的转染降低了 MYB 的 mRNA 和蛋白表达。MYB 的过表达降低了 bta-miR-200b 对牛子宫内膜上皮细胞凋亡的影响。我们的研究结果表明，bta-miR-200b 可以通过靶向 MYB 基因影响牛子宫内膜上皮细胞的凋亡。
