乙酸盐和葡萄糖对雨生红球藻混养中碳固定和碳利用的影响。
The effects of acetate and glucose on carbon fixation and carbon utilization in mixotrophy of Haematococcus pluvialis.
机构信息
Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
Department of Chemical and Biological Engineering, Korea University, 145, Anam-ro, Seoungbuk-gu, Seoul 02841, Republic of Korea.
出版信息
Bioresour Technol. 2023 Jan;367:128218. doi: 10.1016/j.biortech.2022.128218. Epub 2022 Nov 2.
The culture method using sodium acetate and glucose, widely used as organic carbon sources in the mixotrophy of Haematococcus pluvialis, was compared with its autotrophy. In the 12-day culture, mixotrophy using sodium acetate and glucose increased by 40.4% and 77.1%, respectively, compared to autotrophy, but the mechanisms for the increasing biomass were different. The analysis of the mechanism was divided into autotrophic and heterotrophic metabolism. The mixotrophy with glucose increased the biomass by directly supplying the substrate and ATP to the TCA cycle while inhibiting photosynthesis. Gene expressions related to glycolysis and carbon fixation pathway were confirmed in autotrophy and mixotrophy with glucose and acetate. The metabolism predicted in the mixotrophy with acetate and glucose was proposed via autotrophic and heterotrophic metabolism analysis. The mechanism of Haematococcus pluvialis under mixotrophic conditions with high CO concentration was confirmed through this study.
以乙酸钠和葡萄糖作为有机碳源的混合营养培养法，在雨生红球藻的混合营养中被广泛应用，本研究将其与自养培养法进行了比较。在为期 12 天的培养中，与自养培养相比，混合营养培养中使用乙酸钠和葡萄糖分别使生物量增加了 40.4%和 77.1%，但增加生物量的机制不同。通过对机制的分析，将其分为自养和异养代谢。葡萄糖的混合营养通过直接向 TCA 循环提供基质和 ATP 来增加生物量，同时抑制光合作用。在自养和以葡萄糖为碳源的混合营养中，证实了与糖酵解和碳固定途径相关的基因表达。通过对乙酸盐和葡萄糖混合营养的自养和异养代谢分析，提出了乙酸盐和葡萄糖混合营养的代谢预测。本研究通过确认高 CO2 浓度下雨生红球藻混合营养条件下的机制，证实了这一理论。
Zotero 插件