Sugimoto Nao, Morita Yoshifumi, Sakai Eri, Yatomi Yutaka, Kurano Makoto
Faculty of Medicine, The University of Tokyo, Tokyo, Japan.
Department of Clinical Laboratory, The University of Tokyo Hospital, Tokyo, Japan.
Prostaglandins Other Lipid Mediat. 2023 Feb;164:106690. doi: 10.1016/j.prostaglandins.2022.106690. Epub 2022 Nov 2.
Bioactive lipids, such as lysophospholipids, ceramides, and eicosanoids and related mediators, have been demonstrated to be involved in inflammation. We aimed to investigate the possible orchestral modulations of these bioactive lipids in human inflammation. We simultaneously measured the urinary levels of lysophospholipids, ceramides, and eicosanoids and related mediators by a liquid chromatography-mass spectrometry method in patients with cystitis and control subjects. The urinary levels of lysophosphatidylcholine, lysophosphatidylethanolamine, sphingosine 1-phosphate, ceramides, prostaglandin (PG)E and its metabolites represented by tetranor-PGEM, several oxylipins, DHA, and lysoPAF were higher in patients with cystitis. Urinary levels of some species of glycerolysophospholipids were highly positively correlated with those of other species of the same glycerolysophospholipids. Cluster analyses revealed that lysophosphatidylcholine was close to a PGE metabolite, lysophosphatidylethanolamine was close to DHA, and sphingosine 1-phosphate and ceramides were close to lysoPAF. The orchestral dynamism of the lipid mediators was observed in the urine of cystitis, suggesting the necessity for simultaneous investigation of lipid mediators for translational research.
生物活性脂质，如溶血磷脂、神经酰胺、类二十烷酸及相关介质，已被证明与炎症有关。我们旨在研究这些生物活性脂质在人类炎症中可能的协同调节作用。我们采用液相色谱-质谱法同时检测了膀胱炎患者和对照受试者尿液中溶血磷脂、神经酰胺、类二十烷酸及相关介质的水平。膀胱炎患者尿液中溶血磷脂酰胆碱、溶血磷脂酰乙醇胺、1-磷酸鞘氨醇、神经酰胺、前列腺素（PG）E及其以四去甲-PGEM为代表的代谢产物、几种氧化脂质、二十二碳六烯酸（DHA）和溶血血小板活化因子（lysoPAF）的水平较高。某些甘油溶血磷脂种类的尿液水平与同一甘油溶血磷脂其他种类的水平高度正相关。聚类分析显示，溶血磷脂酰胆碱与PGE代谢产物接近，溶血磷脂酰乙醇胺与DHA接近，1-磷酸鞘氨醇和神经酰胺与lysoPAF接近。在膀胱炎患者尿液中观察到脂质介质的协同动态变化，这表明在转化研究中同时研究脂质介质的必要性。
